Genome location: Pf3D7_12_v3:2,105,871..2,108,564(-)
Genome classification: Core
Product Description: tryptophan--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 6.1.1.2 (Tryptophan--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004830 | tryptophan-tRNA ligase activity |
| Process | GO:0009410 | response to xenobiotic stimulus |
| Process | GO:0006436 | tryptophanyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.12 | 0.94 |
| Trophozoite | expressed | 0.38 | 0.77 |
| Schizont | possibly expressed | 0.06 | 0.96 |
| Gametocyte | not expressed | 0.47 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1251700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.145 | MFS: -2.834 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A144A140
"Best" AlphaFill ligand hit: ANL (aniline, Local RMSD=0.42) with 2OV4 (Global RMSD=2.61)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.2 | tryptophan-tRNA ligase | NEMdATPadenineadenosinetryptamineindolmycinL-tryptophanD-tryptophanchuangxinmycinsuccinylacetone3'-O-methyl-ATP5-Methyltryptophan... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101334
Most Similar Human Ortholog: Q9UGM6
TM-align score: 0.84 | RMSD: 2.67
Seq Identity: 0.23 | Length: 332 / 559
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000116874 | tryptophanyl tRNA synthetase 2, mitochondrial |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 29 | 23 | 36 |
| disruptive | 14 | 68 | 34 | 94 |
| missense | 10 | 67 | 34 | 92 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 61 | 24 | 38 |
| disruptive | 28 | 155 | 68 | 147 |
| missense | 21 | 141 | 57 | 112 |
PlasmoDB Total SNPs: 129
Non-coding: 88 | Synonymous: 25 | Nonsynonymous: 16 | Stop Codon: 0
Protein Length: 559 | Molecular Weight (kDa): 66.191
UniProt ID(s): A0A144A140
PDB ID(s): None
Isoelectric Point: 10.02
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001412 | Aminoacyl-tRNA synthetase, class I, conserved site |
| InterPro | IPR002305 | Aminoacyl-tRNA synthetase, class Ic |
| PFam | PF00579 | Aminoacyl-tRNA synthetase, class Ic |
| Superfamily | SSF52374 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 27277538 | Selective inhibition of apicoplast tryptophanyl-tRNA synthetase causes delayeddeath in Plasmodium falciparum. | Pasaje CF, Cheung V, ..., Ralph SA | 10.1038/srep27531 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |