Genome location: Pf3D7_12_v3:2,113,980..2,123,174(-)
Genome classification: Core
Product Description: rhoptry neck protein 3
SignalP Peptide: MNKYWLYIAYVYLLLNILSKC
# Transmembrane Domains: 3
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0005634 | nucleus |
| Component | GO:0020008 | rhoptry |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0003723 | RNA binding |
| Function | GO:0046812 | host cell surface binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
| Process | GO:0030581 | symbiont intracellular protein transport in host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.13 | 0.94 |
| Trophozoite | expressed | 0.24 | 0.88 |
| Schizont | expressed | 3.11 | 0.15 |
| Gametocyte | not expressed | 0.06 | 0.96 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1252100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.175 | MFS: -3.267 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1464900)
Modification: Tagged | RMgm-4716
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4R5
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.59) with 6JWQ (Global RMSD=12.45)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 70 | 38 | 94 |
| disruptive | 48 | 86 | 45 | 140 |
| missense | 47 | 84 | 41 | 121 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 28 | 109 | 65 | 110 |
| disruptive | 72 | 173 | 99 | 225 |
| missense | 62 | 138 | 84 | 162 |
PlasmoDB Total SNPs: 310
Non-coding: 203 | Synonymous: 60 | Nonsynonymous: 47 | Stop Codon: 0
Protein Length: 2215 | Molecular Weight (kDa): 263.155
UniProt ID(s): Q8I4R5
PDB ID(s): None
Isoelectric Point: 9.62
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21237287 | Plasmodial ortholog of Toxoplasma gondii rhoptry neck protein 3 is localized tothe rhoptry body. | Ito D, Han ET, ..., Tsuboi T | 10.1016/j.parint.2011.01.001 |
| 25495792 | PfRON3 is an erythrocyte-binding protein and a potential blood-stage vaccinecandidate antigen. | Zhao X, Chang Z, ..., Chen Q | 10.1186/1475-2875-13-490 |
| 26629826 | Malaria Parasite-Infected Erythrocytes Secrete PfCK1, the Plasmodium Homologue ofthe Pleiotropic Protein Kinase Casein Kinase 1. | Dorin-Semblat D, Demarta-Gatsi C, ..., Doerig C | 10.1371/journal.pone.0139591 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31289187 | Deletion of Plasmodium falciparum Protein R ON3 Affects the Functional Translocation of Exported Proteins and Glucose Uptake | Low LM, Azasi Y, ..., Miller LH | 10.1128/mBio.01460-19 |
| 33537242 | Identification of a Novel RAMA/RON3 Rhoptry Protein Complex in Plasmodiumfalciparum Merozoites. | Ito D, Chen JH, ..., Tsuboi T | 10.3389/fcimb.2020.605367 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 31552198 | Expression and Localization Profiles of Rhoptry Proteins in Plasmodium bergheiSporozoites | Tokunaga N, Nozaki M, ..., Ishino T | 10.3389/fcimb.2019.00316 |
| 26518334 | Variation detection based on next-generation sequencing of type Chinese 1 strainsof Toxoplasma gondii with different virulence from China | Cheng W, Liu F, ..., Shen J | 10.1186/s12864-015-2106-z |
| 30995911 | Hospital-derived antibody profiles of malaria patients in Southwest India | Venkatesh A, Jain A, ..., Rathod PK | 10.1186/s12936-019-2771-5 |
| 37457963 | Roles of the RON3 C-terminal fragment in erythrocyte invasion and blood-stageparasite proliferation in Plasmodium falciparum | Ito D, Kondo Y, ..., Otsuki H | 10.3389/fcimb.2023.1197126 |