Genome location: Pf3D7_12_v3:2,125,435..2,126,571(+)
Genome classification: Core
Product Description: chitinase
SignalP Peptide: MNFTVKYSFLVICLLCCLLSTYVSVIEG
# Transmembrane Domains: 1
EC Numbers: 3.2.1.14 (Chitinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020009 | microneme |
| Function | GO:0008061 | chitin binding |
| Function | GO:0004568 | chitinase activity |
| Process | GO:0006032 | chitin catabolic process |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.07 | 0.96 |
| Trophozoite | not expressed | 0.01 | 1.00 |
| Schizont | not expressed | 0.05 | 0.97 |
| Gametocyte | expressed | 0.01 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1252200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.131 | MFS: -3.146 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0800500)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0800500)
Modification: Mutated | RMgm-4934
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4R4
"Best" AlphaFill ligand hit: CFF (3,7-dihydro-1,3,7-trimethyl-1h-purine-2,6-dione, Local RMSD=0.24) with 3G6M (Global RMSD=8.03)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.2.1.14 | chitinase | SDSKCNDTTEDTANH4+EGTAPMSFHgCl2CaCl2CuSO4ZnSO4PO43-... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 11 | 11 | 19 |
| disruptive | 2 | 10 | 7 | 23 |
| missense | 2 | 10 | 7 | 18 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 29 | 19 | 20 |
| disruptive | 2 | 40 | 29 | 88 |
| missense | 2 | 32 | 24 | 56 |
PlasmoDB Total SNPs: 7
Non-coding: 0 | Synonymous: 4 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 378 | Molecular Weight (kDa): 42.787
UniProt ID(s): Q8I4R4, Q9NJX8, Q9U4I9
PDB ID(s): None
Isoelectric Point: 5.02
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001223 | Glycoside hydrolase family 18, catalytic domain |
| InterPro | IPR011583 | Chitinase II |
| InterPro | IPR017853 | Glycoside hydrolase superfamily |
| PFam | PF00704 | Glycoside hydrolase family 18, catalytic domain |
| Superfamily | SSF51445 | Glycoside hydrolase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10570198 | The chitinase PfCHT1 from the human malaria parasite Plasmodium falciparum lacksproenzyme and chitin-binding domains and displays unique substrate preferences. | Vinetz JM, Dave SK, ..., Fidock DA | 10.1073/pnas.96.24.14061 |
| 11349075 | Disruption of Plasmodium falciparum chitinase markedly impairs parasite invasionof mosquito midgut. | Tsai YL, Hayward RE, ..., Vinetz JM | 10.1128/IAI.69.6.4048-4054.2001 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 19206106 | Characterisation of Plasmodium invasive organelles; an ookinete micronemeproteome. | Lal K, Prieto JH, ..., Sinden RE | 10.1002/pmic.200800404 |