PF3D7_1252600

Genome location: Pf3D7_12_v3:2,147,509..2,152,520(+)

Genome classification: Core

Function and Localization

Product Description: esterase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.-.- (Acting on ester bonds.);3.1.1.5 (Lysophospholipase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft
Component	GO:0016020	membrane
Function	GO:0016788	hydrolase activity, acting on ester bonds
Function	GO:0016298	lipase activity
Process	GO:0006629	lipid metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.36	0.82
Trophozoite	expressed	0.09	0.94
Schizont	expressed	0.05	0.97
Gametocyte	not expressed	0.03	0.98

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1252600

Resistome Missense Mutations: K74N

Resistome Compounds with Missense Mutations: ACT-451840, DDD107498

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -3.203 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1220300)

Relative Growth Rate: 1.09 ± 0.18
Confidence: 4.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I4R0

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.-.-	Ex: 5-phospho-D-xylono-1,4-lactonase	paraoxonatropineethylene terephthalatediisopropyl fluorophosphonatebis(2-hydroxyethyl) terephthalic acidphosphoric acid-bis-4-nitrophenyl ester
3.1.1.5	lysophospholipase	NEMSDSEDTAPCMBEGTAPMSFDMSOCaCl2FeCl3xyleneethanolsulfite...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100231

Most Similar Human Ortholog: A0A3B3ITT3

TM-align score: 0.88 | RMSD: 2.14

Seq Identity: 0.21 | Length: 252 / 453

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000074416	monoglyceride lipase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	18	9	16
disruptive	7	8	12	32
missense	7	8	10	17

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	32	15	18
disruptive	8	58	58	185
missense	8	39	42	86

PlasmoDB Total SNPs: 176

Non-coding: 146 | Synonymous: 23 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 453 | Molecular Weight (kDa): 52.892

UniProt ID(s): Q8I4R0

PDB ID(s): None

Isoelectric Point: 5.16

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006494	Plasmodium subtelomeric PST-A
InterPro	IPR022742	Serine aminopeptidase, S33
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF12146	Serine aminopeptidase, S33
Superfamily	SSF53474	Alpha/Beta hydrolase fold

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
31631203	A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum.	Das D, Krishnan SR, Roy A, Bulusu G	10.1039/c9mo00124g
31772212	Functional annotation of serine hydrolases in the asexual erythrocytic stage ofPlasmodium falciparum.	Elahi R, Ray WK, ..., Klemba M	10.1038/s41598-019-54009-0