Genome location: Pf3D7_12_v3:2,179,909..2,182,869(+)
Genome classification: SubtelomericHypervariable
Product Description: acyl-CoA synthetase
SignalP Peptide: MSIILTFLALFVFLICVFPCIT
# Transmembrane Domains: 0
EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0016020 | membrane |
| Function | GO:0030506 | ankyrin binding |
| Function | GO:0004467 | long-chain fatty acid-CoA ligase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.01 | 1.00 |
| Trophozoite | expressed | 0.13 | 0.90 |
| Schizont | expressed | 0.04 | 0.97 |
| Gametocyte | possibly expressed | 0.00 | 1.00 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1253400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.772 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I0X2
"Best" AlphaFill ligand hit: DYD ((2s,5s)-hexane-2,5-diol, Local RMSD=0.24) with 6Q2M (Global RMSD=3.79)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.3 | long-chain-fatty-acid-CoA ligase | ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100152
Most Similar Human Ortholog: B7Z3Z9
TM-align score: 0.91 | RMSD: 2.62
Seq Identity: 0.23 | Length: 516 / 816
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000151726 | acyl-CoA synthetase long chain family member 1 |
| ENSG00000164398 | acyl-CoA synthetase long chain family member 6 |
| ENSG00000197142 | acyl-CoA synthetase long chain family member 5 |
| ENSG00000281938 | novel transcript |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 103
Non-coding: 13 | Synonymous: 56 | Nonsynonymous: 32 | Stop Codon: 2
Protein Length: 816 | Molecular Weight (kDa): 93.866
UniProt ID(s): Q8I0X2
PDB ID(s): None
Isoelectric Point: 8.1
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000873 | AMP-dependent synthetase/ligase |
| InterPro | IPR020845 | AMP-binding, conserved site |
| PFam | PF00501 | AMP-dependent synthetase/ligase |
| Superfamily | SSF56801 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12554091 | The Plasmodium falciparum fatty acyl-CoA synthetase family (PfACS) anddifferential stage-specific expression in infected erythrocytes. | Matesanz F, Tellez Md, Alcina A | 10.1016/s0166-6851(02)00242-6 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |