Genome location: Pf3D7_12_v3:2,241,271..2,248,962(-)
Genome classification: SubtelomericHypervariable
Product Description: erythrocyte membrane protein 1, PfEMP1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0020030 | infected host cell surface knob |
| Function | GO:0050839 | cell adhesion molecule binding |
| Function | GO:0046789 | host cell surface receptor binding |
| Function | GO:0005178 | integrin binding |
| Process | GO:0020033 | antigenic variation |
| Process | GO:0098609 | cell-cell adhesion |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
| Process | GO:0020013 | modulation by symbiont of host erythrocyte aggregation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.01 | 0.99 |
| Trophozoite | expressed | 0.01 | 1.00 |
| Schizont | expressed | 0.03 | 0.98 |
| Gametocyte | expressed | 0.01 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1255200
Resistome Missense Mutations: T259N, P1331H, R1529T, N2027K
Resistome Compounds with Missense Mutations: AN13762, GNF707, Halofuginone
Resistome # Samples with Disruptive Mutations: 6 (5 missense, 3 "interesting" missense)
Zhang Phenotype: N/A
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I4N5
"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=1.74) with 5MZA (Global RMSD=5.06)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 2609
Non-coding: 401 | Synonymous: 587 | Nonsynonymous: 1607 | Stop Codon: 14
Protein Length: 2268 | Molecular Weight (kDa): 255.417
UniProt ID(s): A0A191VZ98, A0A5P1A059, A0A5P1A088, A0A5P1A0D1, A0A5P1A0F6, A0A5P1A1G6, A0A5P1A1T3, A0A5P1A208, A0A5P1A265, A0A5P1A2M2, A0A5P1A2Z3, A0A5P1A307, A3R6U8, I2CIX8, O61077, Q8I4N5
PDB ID(s): None
Isoelectric Point: 4.76
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004258 | Duffy-binding-like domain |
| InterPro | IPR008602 | Duffy-antigen binding |
| InterPro | IPR029210 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| InterPro | IPR029211 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| InterPro | IPR041480 | Cysteine-rich interdomain region 1 gamma |
| PFam | PF03011 | Duffy-binding-like domain |
| PFam | PF05424 | Duffy-antigen binding |
| PFam | PF15445 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| PFam | PF15447 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| PFam | PF18562 | Cysteine-rich interdomain region 1 gamma |
| Superfamily | SSF140924 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9230440 | P. falciparum rosetting mediated by a parasite-variant erythrocyte membraneprotein and complement-receptor 1. | Rowe JA, Moulds JM, Newbold CI, Miller LH | 10.1038/40888 |
| 9291676 | Knob proteins in falciparum malaria. | Sharma YD | https://pubmed.ncbi.nlm.nih.gov/9291676/ |
| 9786187 | A recombinant peptide based on PfEMP-1 blocks and reverses adhesion ofmalaria-infected red blood cells to CD36 under flow. | Cooke BM, Nicoll CL, Baruch DI, Coppel RL | 10.1046/j.1365-2958.1998.01040.x |
| 11316367 | Variant antigens of Plasmodium falciparum encoded by the var multigenic familyare multifunctional macromolecules. | Nogueira PA, Wunderlich G, Pereira da Silva LH | 10.1016/s0923-2508(01)01184-6 |
| 11420100 | Molecules on the surface of the Plasmodium falciparum infected erythrocyte andtheir role in malaria pathogenesis and immune evasion. | Craig A, Scherf A | https://pubmed.ncbi.nlm.nih.gov/11420100/ |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18785843 | Antigenic variation in Plasmodium falciparum. | Scherf A, Lopez-Rubio JJ, Riviere L | 10.1146/annurev.micro.61.080706.093134 |
| 30552383 | Plasmodium falciparum infected erythrocytes can bind to host receptors integrinsalphaVbeta3 and alphaVbeta6 through DBLdelta1_D4 domain of PFL2665c PfEMP1 protein. | Chesnokov O, Merritt J, ..., Oleinikov AV | 10.1038/s41598-018-36071-2 |