Product Description: erythrocyte binding antigen-140
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020009 | microneme |
| Component | GO:0005886 | plasma membrane |
| Function | GO:0008201 | heparin binding |
| Function | GO:0046789 | host cell surface receptor binding |
| Process | GO:0098609 | cell-cell adhesion |
| Process | GO:0050900 | leukocyte migration |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.88 | 0.75 |
| Trophozoite | expressed | 0.13 | 0.93 |
| Schizont | expressed | 0.56 | 0.82 |
| Gametocyte | possibly expressed | 0.05 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1301600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.952 | #Insertions: 3
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1332700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_1337400)
Modification: Mutated | RMgm-5360
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q76NM5
"Best" AlphaFill ligand hit: 3PO (triphosphate, Local RMSD=0.74) with 5MZA (Global RMSD=6.12)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 30 | 18 | 51 |
| disruptive | 21 | 81 | 47 | 116 |
| missense | 21 | 81 | 47 | 102 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 61 | 31 | 51 |
| disruptive | 36 | 165 | 65 | 179 |
| missense | 32 | 153 | 58 | 146 |
PlasmoDB Total SNPs: 138
Non-coding: 91 | Synonymous: 22 | Nonsynonymous: 24 | Stop Codon: 1
Protein Length: 1210 | Molecular Weight (kDa): 140.596
UniProt ID(s): A0A0K2RVN8, A0A0K2RVS6, A0A159SJ84, A0A159SKR4, A0A159SL22, A0A5P1A0D7, Q105S1, Q105S3, Q105T5, Q76NM5, Q8I049, Q8I787, Q8MM32, Q8MTI6, Q8MTI7, Q8MTI8, Q8MTI9, Q8T9N3, Q8T9Z8, Q8WS31, Q967Z3, Q967Z4
PDB ID(s): None
Isoelectric Point: 6.44
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR008602 | Duffy-antigen binding |
| InterPro | IPR021620 | Erythrocyte binding antigen 175, C-terminal |
| PFam | PF05424 | Duffy-antigen binding |
| PFam | PF11556 | Erythrocyte binding antigen 175, C-terminal |
| Superfamily | SSF140924 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11454199 | A novel ligand from Plasmodium falciparum that binds to a sialic acid-containingreceptor on the surface of human erythrocytes. | Thompson JK, Triglia T, Reed MB, Cowman AF | 10.1046/j.1365-2958.2001.02484.x |
| 12576308 | Glycophorin C is the receptor for the Plasmodium falciparum erythrocyte bindingligand PfEBP-2 (baebl). | Lobo CA, Rodriguez M, Reid M, Lustigman S | 10.1182/blood-2002-10-3076 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 17676953 | Epigenetic silencing of Plasmodium falciparum genes linked to erythrocyteinvasion. | Cortes A, Carret C, ..., Holder AA | 10.1371/journal.ppat.0030107 |
| 19204093 | Polymorphisms in erythrocyte binding antigens 140 and 181 affect function andbinding but not receptor specificity in Plasmodium falciparum. | Maier AG, Baum J, ..., Cowman AF | 10.1128/IAI.01331-08 |
| 22989878 | Crystal and solution structures of Plasmodium falciparum erythrocyte-bindingantigen 140 reveal determinants of receptor specificity during erythrocyteinvasion. | Lin DH, Malpede BM, Batchelor JD, Tolia NH | 10.1074/jbc.M112.409276 |
| 23508963 | Molecular basis for sialic acid-dependent receptor recognition by the Plasmodiumfalciparum invasion protein erythrocyte-binding antigen-140/BAEBL. | Malpede BM, Lin DH, Tolia NH | 10.1074/jbc.M113.450643 |
| 24212193 | Analyses of interactions between heparin and the apical surface proteins ofPlasmodium falciparum. | Kobayashi K, Takano R, ..., Kato K | 10.1038/srep03178 |
| 30642904 | Moderately Neutralizing Epitopes in Nonfunctional Regions Dominate the Antibody Response to Plasmodium falciparum E BA-140 | Salinas ND, Paing MM, ..., Tolia N | 10.1128/IAI.00716-18 |
| 25288451 | Intrinsic disorder within the erythrocyte binding-like proteins from Plasmodiumfalciparum | Blanc M, Coetzer TL, ..., Jensen MR | 10.1016/j.bbapap.2014.09.023 |
| 25588042 | The baculovirus-expressed binding region of Plasmodium falciparum EBA-140 ligandand its glycophorin C binding specificity | Rydzak J, Kaczmarek R, ..., Jaskiewicz E | 10.1371/journal.pone.0115437 |
| 28787025 | Acquisition, maintenance and adaptation of invasion inhibitory antibodies againstPlasmodium falciparum invasion ligands involved in immune evasion | Tijani MK, Babalola OA, ..., Persson KEM | 10.1371/journal.pone.0182187 |
| 25552259 | The structures of glycophorin C N-glycans, a putative component of the GPCreceptor site for Plasmodium falciparum EBA-140 ligand | Ashline DJ, Duk M, ..., Jaskiewicz E | 10.1093/glycob/cwu188 |
| 37754682 | Associations between prenatal malaria exposure, maternal antibodies at birth, andmalaria susceptibility during the first year of life in Burkina Faso | Natama HM, Moncunill G, ..., Dobano C | 10.1128/iai.00268-23 |
| 29115972 | Plasmodium reichenowi EBA-140 merozoite ligand binds to glycophorin D onchimpanzee red blood cells, shedding new light on origins of Plasmodiumfalciparum | Zerka A, Kaczmarek R, Czerwinski M, Jaskiewicz E | 10.1186/s13071-017-2507-8 |
| 26584850 | Erratum to: Studies on Immunogenicity and Antigenicity of Baculovirus-ExpressedBinding Region of Plasmodium falciparum EBA-140 Merozoite Ligand | Zerka A, Rydzak J, ..., Jaskiewicz E | 10.1007/s00005-015-0374-6 |
| 26439848 | Studies on Immunogenicity and Antigenicity of Baculovirus-Expressed BindingRegion of Plasmodium falciparum EBA-140 Merozoite Ligand | Zerka A, Rydzak J, ..., Jaskiewicz E | 10.1007/s00005-015-0367-5 |
| 27443851 | Baculovirus-expressed Plasmodium reichenowi EBA-140 merozoite ligand is hostspecific | Zerka A, Olechwier A, ..., Jaskiewicz E | 10.1016/j.parint.2016.07.009 |
| 25870227 | Variation in Plasmodium falciparum erythrocyte invasion phenotypes and merozoiteligand gene expression across different populations in areas of malariaendemicity | Bowyer PW, Stewart LB, ..., Conway DJ | 10.1128/IAI.03009-14 |
| 30735814 | Few Plasmodium falciparum merozoite ligand and erythrocyte receptor pairs showevidence of balancing selection | Ochola-Oyier LI, Wamae K, ..., Marsh K | 10.1016/j.meegid.2019.02.004 |
| 36812236 | Effects of anti-malarial prophylaxes on maternal transfer of Immunoglobulin-G(IgG) and association to immunity against Plasmodium falciparum infections amongchildren in a Ugandan birth cohort | Okek EJ, Ocan M, ..., Kamya MR | 10.1371/journal.pone.0277789 |
| 35711667 | A Malaria Parasite Cross Reveals Genetic Determinants of Plasmodium falciparumGrowth in Different Culture Media | Kumar S, Li X, ..., Anderson TJC | 10.3389/fcimb.2022.878496 |
| 31234897 | Erythrocyte glycophorins as receptors for Plasmodium merozoites | Jaskiewicz E, Jodlowska M, Kaczmarek R, Zerka A | 10.1186/s13071-019-3575-8 |
| 31409398 | RTS,S/AS01E immunization increases antibody responses to vaccine-unrelatedPlasmodium falciparum antigens associated with protection against clinicalmalaria in African children: a case-control study | Dobano C, Ubillos I, ..., Moncunill G | 10.1186/s12916-019-1378-6 |
| 29128519 | Genetic structure of two erythrocyte binding antigens of Plasmodium falciparumreveals a contrasting pattern of selection | Chowdhury P, Sen S, Kanjilal SD, Sengupta S | 10.1016/j.meegid.2017.11.006 |
| 37090581 | Plasmodium falciparum exploits CD44 as a co-receptor for erythrocyte invasion | Baro-Sastre B, Kim CY, ..., Egan ES | 10.1101/2023.04.12.536503 |
| 28680086 | Host age and expression of genes involved in red blood cell invasion inPlasmodium falciparum field isolates | Valmaseda A, Bassat Q, ..., Mayor A | 10.1038/s41598-017-05025-5 |
| 37832027 | Plasmodium falciparum exploits CD44 as a coreceptor for erythrocyte invasion | Baro B, Kim CY, ..., Egan ES | 10.1182/blood.2023020831 |