Product Description: protein UIS3
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020005 | symbiont-containing vacuole membrane |
| Function | GO:0005515 | protein binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | not expressed | 0.21 | 0.90 |
| Trophozoite | not expressed | 0.04 | 0.98 |
| Schizont | not expressed | 0.04 | 0.97 |
| Gametocyte | not expressed | 0.12 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1302200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.583 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1400800)
Modification: Disrupted | RMgm-4988
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEU1
"Best" AlphaFill ligand hit: PTY (phosphatidylethanolamine, Local RMSD=0.14) with 2VWA (Global RMSD=0.47)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 10 | 3 | 3 |
| disruptive | 6 | 17 | 9 | 24 |
| missense | 6 | 14 | 9 | 21 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 13 | 10 | 10 |
| disruptive | 9 | 43 | 20 | 56 |
| missense | 9 | 35 | 17 | 34 |
PlasmoDB Total SNPs: 50
Non-coding: 30 | Synonymous: 12 | Nonsynonymous: 8 | Stop Codon: 0
Protein Length: 229 | Molecular Weight (kDa): 26.831
UniProt ID(s): Q8IEU1, Q9NFU2, Q9NFU4
PDB ID(s): 2VWA
Isoelectric Point: 9.67
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006389 | Early transcribed membrane protein (ETRAMP) |
| InterPro | IPR021626 | Plasmodium falciparum UIS3 membrane protein |
| PFam | PF09716 | Early transcribed membrane protein (ETRAMP) |
| PFam | PF11567 | Plasmodium falciparum UIS3 membrane protein |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12686607 | etramps, a new Plasmodium falciparum gene family coding for developmentallyregulated and highly charged membrane proteins located at the parasite-host cellinterface. | Spielmann T, Fergusen DJ, Beck HP | 10.1091/mbc.e02-04-0240 |
| 29109477 | Plasmodium UIS3 sequesters host LC3 to avoid elimination by autophagy inhepatocytes. | Real E, Rodrigues L, ..., Mota MM | 10.1038/s41564-017-0054-x |
| 18466298 | Targeted deletion of SAP1 abolishes the expression of infectivity factorsnecessary for successful malaria parasite liver infection | Aly AS, Mikolajczak SA, ..., Kappe SH | 10.1111/j.1365-2958.2008.06271.x |
| 15922944 | Genetically modified Plasmodium highlights the potential of whole parasitevaccine strategies | Good MF | 10.1016/j.it.2005.04.005 |
| 23169100 | Evidence from NMR interaction studies challenges the hypothesis of direct lipidtransfer from L-FABP to malaria sporozoite protein UIS3 | Favretto F, Assfalg M, Molinari H, D'Onofrio M | 10.1002/pro.2194 |
| 21787828 | Comparative efficacy of pre-erythrocytic whole organism vaccine strategiesagainst the malaria parasite | Friesen J, Matuschewski K | 10.1016/j.vaccine.2011.07.034 |
| 33214643 | Disrupting Plasmodium UIS3-host LC3 interaction with a small molecule causesparasite elimination from host cells | Setua S, Enguita FJ, ..., Mota MM | 10.1038/s42003-020-01422-1 |
| 31936739 | Synthetic DNA Vaccines Adjuvanted with pIL-33 Drive Liver-Localized T Cells andProvide Protection from Plasmodium Challenge in a Mouse Model | Reeder SM, Reuschel EL, ..., Aly ASI | 10.3390/vaccines8010021 |
| 35214758 | An Attenuated HSV-1-Derived Malaria Vaccine Expressing Liver-Stage ExportedProteins Induces Sterilizing Protection against Infectious Sporozoite Challenge | Rider PJF, Kamil M, ..., Aly ASI | 10.3390/vaccines10020300 |
| 28031267 | Assessment of the Plasmodium falciparum Preerythrocytic Antigen UIS3 as aPotential Candidate for a Malaria Vaccine | Longley RJ, Halbroth BR, ..., Spencer AJ | 10.1128/IAI.00641-16 |
| 27241521 | Protective efficacy and safety of liver stage attenuated malaria parasites | Kumar H, Sattler JM, ..., Frischknecht F | 10.1038/srep26824 |
| 17303141 | L-FABP is a critical host factor for successful malaria liver stage development | Mikolajczak SA, Jacobs-Lorena V, ..., Kappe SH | 10.1016/j.ijpara.2007.01.002 |
| 23840634 | The ETRAMP family member SEP2 is expressed throughout Plasmodium berghei lifecycle and is released during sporozoite gliding motility | Curra C, Di Luca M, ..., Ponzi M | 10.1371/journal.pone.0067238 |
| 33771680 | Plasmodium UIS3 avoids host cell-autonomous exclusion that requires GABARAPs butnot LC3 and autophagy | Pradipta A, Bando H, ..., Yamamoto M | 10.1016/j.parint.2021.102335 |
| 17624848 | Protracted sterile protection with Plasmodium yoelii pre-erythrocytic geneticallyattenuated parasite malaria vaccines is independent of significant liver-stagepersistence and is mediated by CD8+ T cells | Tarun AS, Dumpit RF, ..., Kappe SH | 10.1086/519742 |
| 22004696 | Immunization with genetically attenuated P52-deficient Plasmodium bergheisporozoites induces a long-lasting effector memory CD8+ T cell response in theliver | Douradinha B, van Dijk M, ..., Epiphanio S | 10.1186/1476-8518-9-6 |
| 18577521 | Crystal structure of soluble domain of malaria sporozoite protein UIS3 in complexwith lipid | Sharma A, Yogavel M, ..., Sharma A | 10.1074/jbc.M801946200 |
| 17591958 | Genetically attenuated Plasmodium berghei liver stages persist and elicit sterileprotection primarily via CD8 T cells | Mueller AK, Deckert M, ..., Schluter D | 10.2353/ajpath.2007.060792 |
| 19214236 | Conserved protective mechanisms in radiation and genetically attenuated uis3(-)and uis4(-) Plasmodium sporozoites | Kumar KA, Baxter P, ..., Nussenzweig V | 10.1371/journal.pone.0004480 |
| 30067422 | Plasmodium protein UIS3 protects the parasite from autophagy clearance | Yao Z, Klionsky DJ | 10.1080/15548627.2018.1483671 |
| 17624847 | Genetically attenuated Plasmodium berghei liver stages induce sterile protractedprotection that is mediated by major histocompatibility complex Class I-dependentinterferon-gamma-producing CD8+ T cells | Jobe O, Lumsden J, ..., Krzych U | 10.1086/519743 |