PF3D7_1302600 (DOHH)

Genome location: Pf3D7_13_v3:142,833..144,141(-)

Genome classification: Core

Function and Localization

Product Description: deoxyhypusine hydroxylase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.14.99.29 (Deoxyhypusine monooxygenase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0016491	oxidoreductase activity
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.36	0.82
Trophozoite	expressed	0.22	0.86
Schizont	expressed	0.05	0.96
Gametocyte	expressed	0.17	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1302600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.133 | MFS: -3.131 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I701

"Best" AlphaFill ligand hit: B3P (2-[3-(2-hydroxy-1,1-dihydroxymethyl-ethylamino)-propylamino]-2-hydroxymethyl-propane-1,3-diol, Local RMSD=1.10) with 4D4Z (Global RMSD=4.22)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.14.99.29	deoxyhypusine monooxygenase	EDTACaCl2ZnCl2MnCl2CuCl2FeSO4FeCl3NiSO4caldinesperminezileutonmimosine...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9BU89	Deoxyhypusine hydroxylase		HOGENOM, OMA, OrthoDB	alpha,alpha'-bipyridineDN-180-01-AFCiclopirox ethanolamineDEFEROXAMINE...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101312

Most Similar Human Ortholog: Q9BU89

TM-align score: 0.87 | RMSD: 2.08

Seq Identity: 0.33 | Length: 283 / 411

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000129932	deoxyhypusine hydroxylase

Protein Information

Protein Length: 411 | Molecular Weight (kDa): 46.451

UniProt ID(s): Q8I701

PDB ID(s): None

Isoelectric Point: 4.72

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004155	PBS lyase HEAT-like repeat
InterPro	IPR016024	Armadillo-type fold
InterPro	IPR027517	Deoxyhypusine hydroxylase
PFam	PF03130	PBS lyase HEAT-like repeat
PFam	PF13646
Superfamily	SSF48371	Armadillo-type fold

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	27	20	28
disruptive	10	62	42	69
missense	9	53	36	62

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	59	17	27
disruptive	25	151	58	138
missense	21	111	48	99

PlasmoDB Total SNPs: 39

Non-coding: 8 | Synonymous: 13 | Nonsynonymous: 17 | Stop Codon: 1

Associated Publications

PMID	Title	Authors	DOI/Link
25715757	New insights into novel inhibitors against deoxyhypusine hydroxylase fromplasmodium falciparum: compounds with an iron chelating potential.	von Koschitzky I, Gerhardt H, ..., Kaiser A	10.1007/s00726-015-1943-z
35007708	Deoxyhypusine hydroxylase as a novel pharmacological target for ischemic strokevia inducing a unique post-translational hypusination modification	Guo Q, Zhang YC, ..., Zeng KW	10.1016/j.phrs.2021.106046
35809341	Biophysical and modeling-based approach for the identification of inhibitorsagainst DOHH from Leishmania donovani	Katiki M, Sharma M, ..., Kumar P	10.1093/bfgp/elac014
37141410	Deoxyhypusine hydroxylase: A novel therapeutic target differentially expressed inshort-term vs long-term survivors of glioblastoma	Ofek P, Yeini E, ..., Satchi-Fainaro R	10.1002/ijc.34545
35403624	[ORF19.2286 Gene: Isolation and Purification of Deoxyhypusine Hydroxylase fromthe Human Pathogenic Yeast Candida albicans]	Agboigba E, Kuchaev E, ..., Validov S	10.31857/S0026898422020021
35458660	Investigation of an Allosteric Deoxyhypusine Synthase Inhibitor in P. falciparum	Aroonsri A, Wongsombat C, ..., Kaiser A	10.3390/molecules27082463