PF3D7_1302700

Genome location: Pf3D7_13_v3:144,689..150,561(+)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase DHR1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Component	GO:0005634	nucleus
Component	GO:0032040	small-subunit processome
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity
Process	GO:0000462	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Process	GO:0042254	ribosome biogenesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	1.17	0.49
Trophozoite	expressed	0.77	0.61
Schizont	possibly expressed	0.20	0.89
Gametocyte	not expressed	0.53	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1302700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.155 | MFS: -2.867 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1401200)

Relative Growth Rate: 0.18 ± 0.16
Confidence: 5.02

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IET8

"Best" AlphaFill ligand hit: MPO (3[n-morpholino]propane sulfonic acid, Local RMSD=0.35) with 6ZM2 (Global RMSD=7.30)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102322

Most Similar Human Ortholog: Q8IY37

TM-align score: 0.64 | RMSD: 6.19

Seq Identity: 0.27 | Length: 896 / 1566

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000150990	DEAH-box helicase 37

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	29	113	55	117
disruptive	126	321	175	320
missense	80	253	145	255

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	56	186	72	115
disruptive	215	639	243	438
missense	123	490	192	268

PlasmoDB Total SNPs: 337

Non-coding: 117 | Synonymous: 98 | Nonsynonymous: 120 | Stop Codon: 2

Protein Information

Protein Length: 1566 | Molecular Weight (kDa): 183.685

UniProt ID(s): Q8IET8

PDB ID(s): None

Isoelectric Point: 8.43

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR002464	DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site
InterPro	IPR007502	Helicase-associated domain
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
PFam	PF04408	Helicase-associated domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase