PF3D7_1303100

Genome location: Pf3D7_13_v3:159,024..160,416(+)

Genome classification: Core

Function and Localization

Product Description: methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.37 (DNA (cytosine-5-)-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0035657	eRF1 methyltransferase complex
Function	GO:0008170	N-methyltransferase activity
Function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
Function	GO:0008276	protein methyltransferase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.10	0.95
Trophozoite	expressed	0.49	0.70
Schizont	expressed	0.09	0.94
Gametocyte	expressed	0.34	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1303100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.135 | MFS: -3.004 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1401600)

Relative Growth Rate: 0.54 ± 0.01
Confidence: 10.00

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1401600)

Modification: Disrupted | RMgm-3728

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IET4

"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=1.80) with 6H1D (Global RMSD=2.96)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.37	DNA (cytosine-5-)-methyltransferase	KClEDTA2'-dCRG108enolasesperminecurcuminNicotineprocainemiR-9-3pmiR-9-5pNSC 14778...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101563

Most Similar Human Ortholog: Q9Y5N5

TM-align score: 0.88 | RMSD: 1.94

Seq Identity: 0.31 | Length: 206 / 222

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000156239	N-6 adenine-specific DNA methyltransferase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	15	13	26
disruptive	6	50	26	57
missense	6	46	25	51

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	34	22	16
disruptive	20	87	50	56
missense	15	77	45	42

PlasmoDB Total SNPs: 100

Non-coding: 60 | Synonymous: 22 | Nonsynonymous: 16 | Stop Codon: 2

Protein Information

Protein Length: 222 | Molecular Weight (kDa): 26.11

UniProt ID(s): Q8IET4

PDB ID(s): None

Isoelectric Point: 7.06

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002052	DNA methylase, N-6 adenine-specific, conserved site
InterPro	IPR007848	Methyltransferase small domain
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF05175	Methyltransferase small domain
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase