About Gene List

PF3D7_1303500 (NHE)

Genome location: Pf3D7_13_v3:169,481..178,156(-)

Genome classification: Core

Function and Localization

Product Description: sodium/hydrogen exchanger

SignalP Peptide: N/A

# Transmembrane Domains: 14

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0016021 integral component of membrane
Component GO:0005886 plasma membrane
Function GO:0015386 potassium:proton antiporter activity
Function GO:0015385 sodium:proton antiporter activity
Function GO:0015299 solute:proton antiporter activity
Process GO:0071805 potassium ion transmembrane transport
Process GO:0051453 regulation of intracellular pH
Process GO:0042493 response to drug
Process GO:0009410 response to xenobiotic stimulus
Process GO:0098719 sodium ion import across plasma membrane
Process GO:0006814 sodium ion transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.69 0.68
Trophozoite expressed 0.68 0.63
Schizont expressed 1.83 0.27
Gametocyte expressed 0.77 0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1303500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 5 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.031 | #Insertions: 9

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IET0

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103045

Most Similar Human Ortholog: C9J3M6

TM-align score: 0.78 | RMSD: 2.09

Seq Identity: 0.12 | Length: 85 / 1920

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000172139 solute carrier family 9 member C1
ENSG00000285044 solute carrier family 9 member C1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 35 127 88 124
disruptive 112 329 142 279
missense 62 222 104 209

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 63 250 94 141
disruptive 208 588 224 414
missense 100 409 158 268

PlasmoDB Total SNPs: 498

Non-coding: 207 | Synonymous: 174 | Nonsynonymous: 117 | Stop Codon: 0

Protein Information

Protein Length: 1920 | Molecular Weight (kDa): 226.083

UniProt ID(s): D9IWH5, D9IWH6, D9IWH7, D9IWH8, D9IWH9, D9IWI1, D9IWI3, D9IWI4, D9IWI5, D9IWI6, D9IWJ0, D9IWJ1, D9IWJ2, D9IWJ4, D9IWJ8, D9IWK0, Q8IET0

PDB ID(s): None

Isoelectric Point: 8.67

Protein Domain Annotations:

Source Family ID Description
InterPro IPR006153 Cation/H+ exchanger
InterPro IPR018422 Cation/H+ exchanger, CPA1 family
PFam PF00999 Cation/H+ exchanger
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
11152613 Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. Krogh A, Larsson B, von Heijne G, Sonnhammer EL 10.1006/jmbi.2000.4315
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
17353059 Plasmodium falciparum Na+/H+ exchanger activity and quinine resistance. Bennett TN, Patel J, Ferdig MT, Roepe PD 10.1016/j.molbiopara.2007.01.018
19428659 Probing the multifactorial basis of Plasmodium falciparum quinine resistance:evidence for a strain-specific contribution of the sodium-proton exchanger PfNHE. Nkrumah LJ, Riegelhaupt PM, ..., Fidock DA 10.1016/j.molbiopara.2009.01.011
21669011 Polymorphism of Plasmodium falciparum Na(+)/H(+) exchanger is indicative of a lowin vitro quinine susceptibility in isolates from Viet Nam. Sinou V, Quang le H, ..., Parzy D 10.1186/1475-2875-10-164