PF3D7_1304400

Genome location: Pf3D7_13_v3:232,308..234,498(-)

Genome classification: Core

Function and Localization

Product Description: PUB domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.30	0.86
Trophozoite	expressed	0.57	0.67
Schizont	expressed	0.25	0.84
Gametocyte	expressed	0.73	0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1304400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.858 | MFS: -2.405 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IES1

"Best" AlphaFill ligand hit: PH2 (2-amino-6-hydroxymethyl-7,8-dihydro-3h-pteridin-4-one, Local RMSD=0.66) with 6JWR (Global RMSD=19.42)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_126530

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	33	25	50
disruptive	17	93	56	116
missense	13	86	54	102

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	75	30	52
disruptive	38	203	76	168
missense	27	176	66	128

PlasmoDB Total SNPs: 75

Non-coding: 24 | Synonymous: 22 | Nonsynonymous: 28 | Stop Codon: 1

Protein Information

Protein Length: 613 | Molecular Weight (kDa): 72.499

UniProt ID(s): Q8IES1

PDB ID(s): None

Isoelectric Point: 6.95

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR018997	PUB domain
InterPro	IPR029071	Ubiquitin-like domain superfamily
InterPro	IPR036339	PUB-like domain superfamily
PFam	PF09409	PUB domain
Superfamily	SSF143503	PUB-like domain superfamily
Superfamily	SSF54236	Ubiquitin-like domain superfamily