Product Description: DNA-directed RNA polymerase II subunit RPB11, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005665 | RNA polymerase II, core complex |
| Component | GO:0016591 | RNA polymerase II, holoenzyme |
| Component | GO:0005634 | nucleus |
| Function | GO:0003899 | DNA-directed 5'-3' RNA polymerase activity |
| Process | GO:0006366 | transcription by RNA polymerase II |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.08 | 0.96 |
| Trophozoite | expressed | 0.14 | 0.91 |
| Schizont | expressed | 0.02 | 0.98 |
| Gametocyte | expressed | 0.04 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1304900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.301 | MFS: 0 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1403400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IER7
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101401
Most Similar Human Ortholog: P52435
TM-align score: 0.90 | RMSD: 1.66
Seq Identity: 0.35 | Length: 115 / 126
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000005075 | RNA polymerase II subunit J |
| ENSG00000228049 | RNA polymerase II subunit J2 |
| ENSG00000267645 | novel protein, POLR2J2-UPK3BL readthrough |
| ENSG00000285437 | RNA polymerase II subunit J3 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 222
Non-coding: 210 | Synonymous: 12 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 126 | Molecular Weight (kDa): 14.125
UniProt ID(s): Q8IER7
PDB ID(s): None
Isoelectric Point: 8.24
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR008193 | DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site |
| InterPro | IPR009025 | DNA-directed RNA polymerase, RBP11-like dimerisation domain |
| InterPro | IPR036603 | RNA polymerase, RBP11-like subunit |
| InterPro | IPR037685 | RNA polymerase RBP11 |
| PFam | PF13656 | DNA-directed RNA polymerase, RBP11-like dimerisation domain |
| Superfamily | SSF55257 | RNA polymerase, RBP11-like subunit |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 15987790 | Distinct regions of RPB11 are required for heterodimerization with RPB3 in humanand yeast RNA polymerase II | Benga WJ, Grandemange S, ..., Vigneron M | 10.1093/nar/gki672 |
| 25549102 | Measurement of mRNA decay rates in Saccharomyces cerevisiae using rpb1-1 strains | Peccarelli M, Kebaara BW | 10.3791/52240 |
| 24727477 | Mediator, TATA-binding protein, and RNA polymerase II contribute to low histoneoccupancy at active gene promoters in yeast | Ansari SA, Paul E, ..., Morse RH | 10.1074/jbc.M113.529354 |
| 16621069 | Characterization of RNA polymerase II subunits of Trypanosoma brucei | Devaux S, Lecordier L, ..., Vanhamme L | 10.1016/j.molbiopara.2006.02.020 |
| 20637075 | RNA polymerase mapping during stress responses reveals widespread nonproductivetranscription in yeast | Kim TS, Liu CL, ..., Rando OJ | 10.1186/gb-2010-11-7-r75 |
| 19928061 | [The functional interaction of an RNA polymerase II Rpb11 subunit with the Med18subunit (Srb5) of the Saccharomyces cerevisiae mediator complex] | Proshkina GM, Shpakovskii GV | 10.1134/s1068162009040153 |
| 18077427 | Protein characterization of Saccharomyces cerevisiae RNA polymerase II after invivo cross-linking | Tardiff DF, Abruzzi KC, Rosbash M | 10.1073/pnas.0710179104 |
| 16537912 | cis- and trans-Acting determinants of transcription termination by yeast RNApolymerase II | Steinmetz EJ, Ng SB, Cloute JP, Brow DA | 10.1128/MCB.26.7.2688-2696.2006 |
| 28665995 | Transcriptomes of six mutants in the Sen1 pathway reveal combinatorial control oftranscription termination across the Saccharomyces cerevisiae genome | Chen X, Poorey K, ..., Brow DA | 10.1371/journal.pgen.1006863 |
| 20944015 | Overlapping regulation of CenH3 localization and histone H3 turnover by CAF-1 andHIR proteins in Saccharomyces cerevisiae | Lopes da Rosa J, Holik J, ..., Kaufman PD | 10.1534/genetics.110.123117 |
| 16507144 | Release of extraction-resistant mRNA in stationary phase Saccharomyces cerevisiaeproduces a massive increase in transcript abundance in response to stress | Aragon AD, Quinones GA, ..., Werner-Washburne M | 10.1186/gb-2006-7-2-r9 |