About Gene List

PF3D7_1305000

Genome location: Pf3D7_13_v3:246,965..252,322(+)

Genome classification: Core

Function and Localization

Product Description: MCL1 domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0043596 nuclear replication fork
Component GO:0005634 nucleus
Component GO:0031298 replication fork protection complex
Function GO:0003682 chromatin binding
Process GO:0006281 DNA repair
Process GO:0006261 DNA-dependent DNA replication
Process GO:0006974 cellular response to DNA damage stimulus
Process GO:0000278 mitotic cell cycle

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.25 0.88
Trophozoite expressed 1.34 0.38
Schizont expressed 0.93 0.55
Gametocyte not expressed 0.79 0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1305000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.247 | MFS: -2.625 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1403500)

  • Relative Growth Rate: 0.08 ± 0.14
  • Confidence: 5.29

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IER6

"Best" AlphaFill ligand hit: BMF (bromosporine, Local RMSD=0.44) with 5U9N (Global RMSD=9.19)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532794

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 14 95 51 105
disruptive 42 165 93 241
missense 38 162 88 220

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 26 180 76 131
disruptive 75 354 196 332
missense 60 317 175 260

PlasmoDB Total SNPs: 170

Non-coding: 46 | Synonymous: 67 | Nonsynonymous: 57 | Stop Codon: 0

Protein Information

Protein Length: 1479 | Molecular Weight (kDa): 174.473

UniProt ID(s): Q8IER6

PDB ID(s): None

Isoelectric Point: 5.62

Protein Domain Annotations:

Source Family ID Description
InterPro IPR022100 Minichromosome loss protein Mcl1, middle region
InterPro IPR036322 WD40-repeat-containing domain superfamily
PFam PF12341 Minichromosome loss protein Mcl1, middle region
Superfamily SSF50978 WD40-repeat-containing domain superfamily

Associated Publications

PMID Title Authors DOI/Link
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108