PF3D7_1305500 (MKP1)

Genome location: Pf3D7_13_v3:270,846..273,772(+)

Genome classification: Core

Function and Localization

Product Description: mitogen-activated protein kinase phosphatase 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 2.7.11.24 (Mitogen-activated protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0007049	cell cycle
Process	GO:0042493	response to drug
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.04	0.98
Trophozoite	no data	0.10	0.93
Schizont	no data	0.01	0.99
Gametocyte	no data	0.13	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1305500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.153 | #Insertions: 30

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1404000)

Relative Growth Rate: 1.00 ± 0.10
Confidence: 5.96

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1404000)

Modification: Disrupted | RMgm-3743

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IER0

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.24	mitogen-activated protein kinase	ADPH89MKK4MKP3MG132U0126SB202VX745siRNAML3403SR3451SR3582...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_172069

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	36	23	56
disruptive	20	114	70	163
missense	17	111	69	147

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	68	35	68
disruptive	37	233	123	196
missense	30	217	112	146

PlasmoDB Total SNPs: 91

Non-coding: 44 | Synonymous: 24 | Nonsynonymous: 23 | Stop Codon: 0

Protein Information

Protein Length: 771 | Molecular Weight (kDa): 92.276

UniProt ID(s): Q8IER0

PDB ID(s): None

Isoelectric Point: 7.3

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001763	Rhodanese-like domain
InterPro	IPR036873	Rhodanese-like domain superfamily
PFam	PF00581	Rhodanese-like domain
Superfamily	SSF52821	Rhodanese-like domain superfamily

PMID	Title	Authors	DOI/Link
23813392	Atypical mitogen-activated protein kinase phosphatase implicated in regulatingtransition from pre-S-Phase asexual intraerythrocytic development of Plasmodiumfalciparum.	Balu B, Campbell C, ..., Adams JH	10.1128/EC.00028-13
24605883	In silico characterization of an atypical MAPK phosphatase of Plasmodiumfalciparum as a suitable target for drug discovery.	Campbell CO, Santiago DN, ..., Adams JH	10.1111/cbdd.12315