PF3D7_1306300

Genome location: Pf3D7_13_v3:292,977..296,617(+)

Genome classification: Core

Function and Localization

Product Description: SAM dependent methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.35 (tRNA (uracil(54)-C(5))-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0030696	tRNA (m5U54) methyltransferase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.05	0.98
Trophozoite	not expressed	0.42	0.76
Schizont	not expressed	0.05	0.97
Gametocyte	not expressed	0.26	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1306300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.192 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1404800)

Relative Growth Rate: 0.92 ± 0.10
Confidence: 5.90

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1404800)

Modification: Disrupted | RMgm-3750

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5A2

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.35	tRNA (uracil54-C5)-methyltransferase	NH4+tRNAPhespermidineputrescinenorleucineL-ethioninemature tRNANO2Ura-tRNAL-methionineribonuclease5-fluorouracilethylthioadenosine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_179351

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	35	17	47
disruptive	19	136	80	168
missense	18	130	78	159

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	61	33	65
disruptive	40	290	112	203
missense	32	266	108	158

PlasmoDB Total SNPs: 171

Non-coding: 90 | Synonymous: 46 | Nonsynonymous: 35 | Stop Codon: 0

Protein Information

Protein Length: 800 | Molecular Weight (kDa): 95.891

UniProt ID(s): C0H5A2

PDB ID(s): None

Isoelectric Point: 9.08

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR010280	(Uracil-5)-methyltransferase family
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
InterPro	IPR030390	RNA methyltransferase TrmA, active site
PFam	PF05958	(Uracil-5)-methyltransferase family
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase