Product Description: 26S protease regulatory subunit 10B, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.8 (Transferred entry: 5.6.1.5)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031597 | cytosolic proteasome complex |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Component | GO:0005838 | proteasome regulatory particle |
| Component | GO:0008540 | proteasome regulatory particle, base subcomplex |
| Function | GO:0004175 | endopeptidase activity |
| Process | GO:0045899 | positive regulation of RNA polymerase II transcription preinitiation complex assembly |
| Process | GO:0070682 | proteasome regulatory particle assembly |
| Process | GO:0030433 | ubiquitin-dependent ERAD pathway |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.31 | 0.85 |
| Trophozoite | expressed | 1.04 | 0.46 |
| Schizont | expressed | 0.72 | 0.59 |
| Gametocyte | expressed | 0.43 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1306400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.136 | MFS: -2.646 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1404900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEQ1
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.14) with 7W46 (Global RMSD=3.83)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.8 | proteasome ATPase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101751
Most Similar Human Ortholog: P62333
TM-align score: 0.97 | RMSD: 1.34
Seq Identity: 0.69 | Length: 387 / 393
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000100519 | proteasome 26S subunit, ATPase 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 119
Non-coding: 109 | Synonymous: 8 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 393 | Molecular Weight (kDa): 44.677
UniProt ID(s): Q8IEQ1
PDB ID(s): None
Isoelectric Point: 9.3
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR003960 | ATPase, AAA-type, conserved site |
| InterPro | IPR005937 | 26S proteasome regulatory subunit P45-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032501 | Proteasomal ATPase OB C-terminal domain |
| InterPro | IPR041569 | AAA ATPase, AAA+ lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF16450 | Proteasomal ATPase OB C-terminal domain |
| PFam | PF17862 | AAA ATPase, AAA+ lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 28784663 | The 19S proteasome is directly involved in the regulation of heterochromatinspreading in fission yeast | Seo HD, Choi Y, ..., Lee D | 10.1074/jbc.M117.790824 |
| 26997367 | Targeting the 19S proteasomal subunit, Rpt4, for the treatment of colon cancer | Boland K, Flanagan L, ..., Prehn JH | 10.1016/j.ejphar.2016.03.031 |
| 29435775 | Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway | Chen K, Rekep M, ..., Liu Y | 10.1007/s10557-018-6771-4 |
| 29214404 | The 19S proteasome regulates subtelomere silencing and facultativeheterochromatin formation in fission yeast | Seo HD, Kwon CS, Lee D | 10.1007/s00294-017-0792-6 |
| 22474342 | Similar temporal and spatial recruitment of native 19S and 20S proteasome subunits to transcriptionally active chromatin | Geng F, Tansey WP | 10.1073/pnas.1200854109 |
| 23562395 | Conformational dynamics of the Rpt6 ATPase in proteasome assembly and Rpn14 binding | Ehlinger A, Park S, ..., Walters KJ | 10.1016/j.str.2013.02.021 |