PF3D7_1306600

Genome location: Pf3D7_13_v3:314,683..316,989(-)

Genome classification: Core

Function and Localization

Product Description: V-type proton ATPase subunit H, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.3.14 (Transferred entry: 7.1.2.2)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0000221	vacuolar proton-transporting V-type ATPase, V1 domain
Function	GO:0046933	proton-transporting ATP synthase activity, rotational mechanism
Function	GO:0046961	proton-transporting ATPase activity, rotational mechanism
Process	GO:0007035	vacuolar acidification

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.17	0.91
Trophozoite	expressed	0.39	0.76
Schizont	expressed	0.19	0.87
Gametocyte	possibly expressed	0.30	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1306600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.254 | MFS: -2.63 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1405100)

Relative Growth Rate: 0.10 ± 1.35
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEP9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.3.14	H+-transporting two-sector ATPase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101800

Most Similar Human Ortholog: E5RK31

TM-align score: 0.76 | RMSD: 1.42

Seq Identity: 0.03 | Length: 33 / 455

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000047249	ATPase H+ transporting V1 subunit H

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	30	14	33
disruptive	4	8	11	24
missense	4	7	11	20

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	66	20	35
disruptive	9	47	30	117
missense	8	35	25	66

PlasmoDB Total SNPs: 101

Non-coding: 73 | Synonymous: 21 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 455 | Molecular Weight (kDa): 53.829

UniProt ID(s): Q8IEP9

PDB ID(s): None

Isoelectric Point: 6.58

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004908	ATPase, V1 complex, subunit H
InterPro	IPR011987	ATPase, V1 complex, subunit H, C-terminal
InterPro	IPR016024	Armadillo-type fold
PFam	PF03224	ATPase, V1 complex, subunit H
PFam	PF11698	ATPase, V1 complex, subunit H, C-terminal
Superfamily	SSF48371	Armadillo-type fold