Product Description: ATP-dependent RNA helicase DBP6, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0030687 | preribosome, large subunit precursor |
| Function | GO:0003724 | RNA helicase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.32 | 0.80 |
| Schizont | possibly expressed | 0.05 | 0.97 |
| Gametocyte | expressed | 0.61 | 0.66 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1307300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.25 | MFS: -3.316 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1405800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEP2
"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.01) with 6L5M (Global RMSD=2.71)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 36 | 18 | 35 |
| disruptive | 6 | 48 | 33 | 88 |
| missense | 5 | 46 | 32 | 81 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 65 | 25 | 50 |
| disruptive | 17 | 112 | 51 | 127 |
| missense | 11 | 98 | 47 | 103 |
PlasmoDB Total SNPs: 85
Non-coding: 39 | Synonymous: 39 | Nonsynonymous: 6 | Stop Codon: 1
Protein Length: 630 | Molecular Weight (kDa): 74.189
PDB ID(s): None
Isoelectric Point: 9.68
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 10567587 | Synthetic lethality with conditional dbp6 alleles identifies rsa1p, anucleoplasmic protein involved in the assembly of 60S ribosomal subunits | Kressler D, Doere M, Rojo M, Linder P | 10.1128/MCB.19.12.8633 |
| 17145778 | Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals alow-molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3pinvolved in early steps of 60S ribosomal subunit biogenesis | Rosado IV, Dez C, ..., de la Cruz J | 10.1128/MCB.01523-06 |
| 15126390 | The putative RNA helicase Dbp6p functionally interacts with Rpl3p, Nop8p and thenovel trans-acting Factor Rsa3p during biogenesis of 60S ribosomal subunits inSaccharomyces cerevisiae | de la Cruz J, Lacombe T, ..., Kressler D | 10.1534/genetics.166.4.1687 |
| 9528757 | Dbp6p is an essential putative ATP-dependent RNA helicase required for60S-ribosomal-subunit assembly in Saccharomyces cerevisiae | Kressler D, de la Cruz J, Rojo M, Linder P | 10.1128/MCB.18.4.1855 |
| 16449635 | Comprehensive mutational analysis of yeast DEXD/H box RNA helicases involved inlarge ribosomal subunit biogenesis | Bernstein KA, Granneman S, ..., Baserga SJ | 10.1128/MCB.26.4.1195-1208.2006 |
| 11565753 | Dbp9p, a putative ATP-dependent RNA helicase involved in 60S-ribosomal-subunitbiogenesis, functionally interacts with Dbp6p | Daugeron MC, Kressler D, Linder P | 10.1017/s1355838201010640 |