Product Description: DNA-directed RNA polymerase subunit alpha, putative
SignalP Peptide: MSLLFLLLFFLTECNIYWS
# Transmembrane Domains: 0
EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Function | GO:0003899 | DNA-directed 5'-3' RNA polymerase activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.20 | 0.90 |
| Trophozoite | expressed | 0.59 | 0.66 |
| Schizont | expressed | 0.54 | 0.68 |
| Gametocyte | not expressed | 0.45 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1307600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.769 | MFS: -2.584 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1406100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEN9
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.6 | DNA-directed RNA polymerase | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P0A7Z4 | DNA-directed RNA polymerase subunit alpha | OrthoMCL BLAST | US10800725, Compound 15US10800725, Compound 19US10800725, Compound 1US10800725, Compound 2... | |
| P9WGZ1 | DNA-directed RNA polymerase subunit alpha | OrthoMCL BLAST | US10800725, Compound 15US10800725, Compound 19US10800725, Compound 20US10800725, Compound 1... | |
| P66706 | DNA-directed RNA polymerase subunit alpha | OrthoMCL BLAST | US10800725, Compound 1US10800725, Compound 2US10800725, Compound 3US10800725, Compound 4... |
Protein Length: 619 | Molecular Weight (kDa): 74.02
UniProt ID(s): Q8IEN9
PDB ID(s): None
Isoelectric Point: 5.98
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011262 | DNA-directed RNA polymerase, insert domain |
| InterPro | IPR011263 | DNA-directed RNA polymerase, RpoA/D/Rpb3-type |
| InterPro | IPR036603 | RNA polymerase, RBP11-like subunit |
| InterPro | IPR036643 | DNA-directed RNA polymerase, insert domain superfamily |
| PFam | PF01000 | DNA-directed RNA polymerase, insert domain |
| Superfamily | SSF55257 | RNA polymerase, RBP11-like subunit |
| Superfamily | SSF56553 | DNA-directed RNA polymerase, insert domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 37 | 17 | 31 |
| disruptive | 6 | 29 | 19 | 49 |
| missense | 6 | 29 | 19 | 45 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 72 | 14 | 28 |
| disruptive | 12 | 82 | 64 | 130 |
| missense | 12 | 66 | 51 | 98 |
PlasmoDB Total SNPs: 134
Non-coding: 98 | Synonymous: 29 | Nonsynonymous: 7 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 37732780 | Insight into the drug-resistant characteristics and genetic diversity of multidrug-resistant Mycobacterium tuberculosis in China | Song Z, Liu C, ..., Zhao Y | 10.1128/spectrum.01324-23 |
| 37721232 | Rifampicin resistance conferring mutations among Mycobacterium tuberculosisstrains in Rwanda | Cuella-Martin I, Semuto Ngabonziza JC, ..., Rigouts L | 10.4103/ijmy.ijmy_103_23 |