PF3D7_1309300 (PRPF3)

Genome location: Pf3D7_13_v3:427,265..430,292(-)

Genome classification: Core

Function and Localization

Product Description: U4/U6 small nuclear ribonucleoprotein PRP3, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0046540	U4/U6 x U5 tri-snRNP complex
Process	GO:0000398	mRNA splicing, via spliceosome

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.09	0.53
Trophozoite	expressed	0.93	0.51
Schizont	expressed	0.55	0.68
Gametocyte	expressed	0.80	0.57

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1309300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -3.064 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1407800)

Relative Growth Rate: 0.05 ± 0.05
Confidence: 7.25

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEM1

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102616

Most Similar Human Ortholog: O43395

TM-align score: 0.44 | RMSD: 4.68

Seq Identity: 0.30 | Length: 348 / 728

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000117360	pre-mRNA processing factor 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	56	23	50
disruptive	28	115	65	172
missense	15	101	59	124

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	24	90	34	59
disruptive	66	260	121	272
missense	36	213	93	147

PlasmoDB Total SNPs: 173

Non-coding: 67 | Synonymous: 62 | Nonsynonymous: 44 | Stop Codon: 0

Protein Information

Protein Length: 728 | Molecular Weight (kDa): 86.49

UniProt ID(s): Q8IEM1

PDB ID(s): None

Isoelectric Point: 9.62

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR010541	Domain of unknown function DUF1115
InterPro	IPR013881	Pre-mRNA-splicing factor 3
InterPro	IPR027104	U4/U6 small nuclear ribonucleoprotein Prp3
PFam	PF06544	Domain of unknown function DUF1115
PFam	PF08572	Pre-mRNA-splicing factor 3
Superfamily	N/A	N/A