PF3D7_1309600

Genome location: Pf3D7_13_v3:438,135..439,566(+)

Genome classification: Core

Function and Localization

Product Description: ribosomal RNA methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.56 (mRNA (guanine-N(7)-)-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0008173	RNA methyltransferase activity
Function	GO:0008650	rRNA (uridine-2'-O-)-methyltransferase activity
Process	GO:0001510	RNA methylation
Process	GO:0006364	rRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.10	0.95
Trophozoite	possibly expressed	0.21	0.87
Schizont	possibly expressed	0.02	0.98
Gametocyte	not expressed	0.22	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1309600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.21 | MFS: -2.754 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1408100)

Relative Growth Rate: 0.14 ± 0.11
Confidence: 5.73

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEL9

"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=0.39) with 2PLW (Global RMSD=1.71)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.56	mRNA (guanine-N7)-methyltransferase	EDTAGpppGThesitribavirinDigitoninsinefunginTriton X-100deoxycholatecycloheximideS-adenosyl-L-cysteineaurintricarboxylic acidS-adenosyl-homocysteine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103441

Most Similar Human Ortholog: Q9UI43

TM-align score: 0.81 | RMSD: 1.98

Seq Identity: 0.30 | Length: 213 / 288

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000122687	mitochondrial rRNA methyltransferase 2
ENSG00000286192	Novel protein

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	16	6	19
disruptive	6	15	19	43
missense	6	15	19	41

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	26	13	27
disruptive	8	56	30	80
missense	7	48	27	60

PlasmoDB Total SNPs: 72

Non-coding: 60 | Synonymous: 9 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 288 | Molecular Weight (kDa): 33.713

UniProt ID(s): Q8IEL9

PDB ID(s): 2PLW

Isoelectric Point: 9.92

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002877	Ribosomal RNA methyltransferase FtsJ domain
InterPro	IPR015507	Ribosomal RNA large subunit methyltransferase E
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF01728	Ribosomal RNA methyltransferase FtsJ domain
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase