PF3D7_1309700 (VPS18)

Genome location: Pf3D7_13_v3:440,240..445,360(+)

Genome classification: Core

Function and Localization

Product Description: vacuolar protein sorting-associated protein 18, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0030897	HOPS complex
Component	GO:0005768	endosome
Function	GO:0030674	protein-macromolecule adaptor activity
Process	GO:0007032	endosome organization
Process	GO:0035542	regulation of SNARE complex assembly
Process	GO:0007033	vacuole organization
Process	GO:0006904	vesicle docking involved in exocytosis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.34	0.84
Trophozoite	expressed	0.54	0.69
Schizont	expressed	0.17	0.89
Gametocyte	expressed	0.54	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1309700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.011 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1408200)

Relative Growth Rate: 0.08 ± 0.04
Confidence: 7.94

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEL8

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102656

Most Similar Human Ortholog: H0YMC9

TM-align score: 0.49 | RMSD: 2.38

Seq Identity: 0.14 | Length: 72 / 1672

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000104142	VPS18 core subunit of CORVET and HOPS complexes

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	24	103	41	134
disruptive	94	296	147	355
missense	59	231	121	287

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	34	191	69	132
disruptive	156	580	226	503
missense	93	449	176	339

PlasmoDB Total SNPs: 169

Non-coding: 3 | Synonymous: 95 | Nonsynonymous: 71 | Stop Codon: 0

Protein Information

Protein Length: 1672 | Molecular Weight (kDa): 198.335

UniProt ID(s): Q8IEL8

PDB ID(s): None

Isoelectric Point: 7.76

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007810	Pep3/Vps18/deep orange
PFam	PF05131	Pep3/Vps18/deep orange
Superfamily	SSF57850	N/A

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
34013567	Homozygous missense VPS16 variant is associated with a novel disease, resemblingmucopolysaccharidosis-plus syndrome in two siblings	Yildiz Y, Kosukcu C, ..., Dursun A	10.1111/cge.14002