Product Description: ras-related protein Rab-5B
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.5.2 (Small monomeric GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0000421 | autophagosome membrane |
| Component | GO:0012505 | endomembrane system |
| Component | GO:0020020 | food vacuole |
| Component | GO:0044310 | osmiophilic body |
| Component | GO:0005886 | plasma membrane |
| Component | GO:0085026 | tubovesicular membrane network |
| Function | GO:0003924 | GTPase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0006886 | intracellular protein transport |
| Process | GO:0043952 | protein transport by the Sec complex |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.01 | 0.99 |
| Trophozoite | expressed | 0.01 | 0.99 |
| Schizont | expressed | 0.07 | 0.94 |
| Gametocyte | expressed | 0.01 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1310600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 2.769 | #Insertions: 2
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1409100)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1409100)
Modification: Mutated | RMgm-5219
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q76NM7
"Best" AlphaFill ligand hit: GNP (phosphoaminophosphonic acid-guanylate ester, Local RMSD=0.02) with 3LAW (Global RMSD=2.65)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.2 | small monomeric GTPase | GTPGDPEDTAPlexAstatinCCG-977Rap1GapNSC23766CCG-1423Rap1GAP1GTPgammaSCalmodulin... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 0 |
| missense | 0 | 0 | 0 | 0 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 0 | 0 | 0 |
| disruptive | 0 | 0 | 0 | 1 |
| missense | 0 | 0 | 0 | 0 |
PlasmoDB Total SNPs: 170
Non-coding: 168 | Synonymous: 1 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 207 | Molecular Weight (kDa): 23.366
PDB ID(s): None
Isoelectric Point: 8.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001806 | Small GTPase |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR025662 | Sigma-54 interaction domain, ATP-binding site 1 |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00071 | Small GTPase |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12657463 | The Plasmodium falciparum family of Rab GTPases. | Quevillon E, Spielmann T, ..., Langsley G | 10.1016/s0378-1119(03)00381-0 |
| 17919451 | Unraveling the components of protein translocation pathway in human malariaparasite Plasmodium falciparum. | Tuteja R | 10.1016/j.abb.2007.08.031 |
| 22188458 | Construction of a Plasmodium falciparum Rab-interactome identifies CK1 and PKA asRab-effector kinases in malaria parasites. | Rached FB, Ndjembo-Ezougou C, ..., Langsley G | 10.1111/boc.201100081 |
| 24498355 | Plasmodium falciparum Rab5B is an N-terminally myristoylated Rab GTPase that istargeted to the parasite's plasma and food vacuole membranes. | Ezougou CN, Ben-Rached F, ..., Langsley G | 10.1371/journal.pone.0087695 |
| 27316546 | Plasmodium Rab5b is secreted to the cytoplasmic face of the tubovesicular networkin infected red blood cells together with N-acylated adenylate kinase 2. | Ebine K, Hirai M, ..., Saito-Nakano Y | 10.1186/s12936-016-1377-4 |
| 27432909 | Comparative Proteomics and Functional Analysis Reveal a Role of Plasmodiumfalciparum Osmiophilic Bodies in Malaria Parasite Transmission. | Suarez-Cortes P, Sharma V, ..., Alano P | 10.1074/mcp.M116.060681 |
| 33604307 | Rab5b-Associated Arf1 GTPase Regulates Export of N-Myristoylated Adenylate Kinase2 From the Endoplasmic Reticulum in Plasmodium falciparum. | Taku I, Hirai T, ..., Saito-Nakano Y | 10.3389/fcimb.2020.610200 |
| 35649358 | Active APPL1 sequestration by Plasmodium favors liver-stage development | Lahree A, Baptista SJS, ..., Mota MM | 10.1016/j.celrep.2022.110886 |