PF3D7_1311400 (AP1M1)

Genome location: Pf3D7_13_v3:487,208..489,812(+)

Genome classification: Core

Function and Localization

Product Description: AP-1 complex subunit mu-1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Component	GO:0030131	clathrin adaptor complex
Component	GO:0030125	clathrin vesicle coat
Component	GO:0031410	cytoplasmic vesicle
Component	GO:0043231	intracellular membrane-bounded organelle
Component	GO:0020008	rhoptry
Function	GO:0005515	protein binding
Process	GO:0006886	intracellular protein transport
Process	GO:0016192	vesicle-mediated transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.37	0.82
Trophozoite	expressed	0.62	0.64
Schizont	expressed	0.33	0.78
Gametocyte	expressed	0.34	0.80

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1311400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.319 | MFS: -3.464 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1409900)

Relative Growth Rate: 0.07 ± 0.21
Confidence: 4.48

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEK4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101263

Most Similar Human Ortholog: K7EJL1

TM-align score: 0.98 | RMSD: 1.19

Seq Identity: 0.62 | Length: 351 / 437

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000072958	adaptor related protein complex 1 subunit mu 1
ENSG00000129354	adaptor related protein complex 1 subunit mu 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	27	15	38
disruptive	0	10	4	20
missense	0	9	4	19

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	56	17	33
disruptive	2	21	22	41
missense	1	17	22	29

PlasmoDB Total SNPs: 165

Non-coding: 148 | Synonymous: 16 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 437 | Molecular Weight (kDa): 50.811

UniProt ID(s): A0A5J6BWG8, A0A5J6BWH5, A0A5J6BX73, A0A5J6BYE4, A0A5J6BZ98, A0A5J6BZE9, A0A5J6BZM0, A0A5J6BZY7, A0A5J6C1J8, Q8IEK4

PDB ID(s): None

Isoelectric Point: 9.47

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001392	Clathrin adaptor, mu subunit
InterPro	IPR011012	Longin-like domain superfamily
InterPro	IPR018240	Clathrin adaptor, mu subunit, conserved site
InterPro	IPR022775	AP complex, mu/sigma subunit
InterPro	IPR028565	Mu homology domain
InterPro	IPR036168	AP-2 complex subunit mu, C-terminal superfamily
PFam	PF00928	Mu homology domain
PFam	PF01217	AP complex, mu/sigma subunit
Superfamily	SSF49447	AP-2 complex subunit mu, C-terminal superfamily
Superfamily	SSF64356	Longin-like domain superfamily