Product Description: 26S protease regulatory subunit 7, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.8 (Transferred entry: 5.6.1.5)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0005838 | proteasome regulatory particle |
| Component | GO:0008540 | proteasome regulatory particle, base subcomplex |
| Function | GO:0017025 | TBP-class protein binding |
| Function | GO:0004175 | endopeptidase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0045899 | positive regulation of RNA polymerase II transcription preinitiation complex assembly |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.16 | 0.92 |
| Trophozoite | expressed | 0.52 | 0.69 |
| Schizont | expressed | 0.52 | 0.67 |
| Gametocyte | expressed | 0.41 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1311500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.924 | MFS: -2.109 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1410000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEK3
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.08) with 6P8V (Global RMSD=4.15)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.8 | proteasome ATPase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101899
Most Similar Human Ortholog: C9JX88
TM-align score: 0.97 | RMSD: 1.20
Seq Identity: 0.74 | Length: 417 / 420
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000161057 | proteasome 26S subunit, ATPase 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 21 | 16 | 25 |
| disruptive | 1 | 0 | 4 | 19 |
| missense | 1 | 0 | 4 | 15 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 49 | 14 | 29 |
| disruptive | 1 | 10 | 15 | 66 |
| missense | 1 | 6 | 14 | 50 |
PlasmoDB Total SNPs: 101
Non-coding: 95 | Synonymous: 5 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 420 | Molecular Weight (kDa): 46.835
UniProt ID(s): Q8IEK3
PDB ID(s): None
Isoelectric Point: 6.63
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR003960 | ATPase, AAA-type, conserved site |
| InterPro | IPR005937 | 26S proteasome regulatory subunit P45-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR041569 | AAA ATPase, AAA+ lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF17862 | AAA ATPase, AAA+ lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30323024 | Plasmodium falciparum Cyclic G MP-Dependent Protein Kinase Interacts with a Subunit of the Parasite Proteasome | Govindasamy K, Khan R, ..., Bhanot P | 10.1128/IAI.00523-18 |
| 32693118 | Partial impairment of late-stage autophagic flux in murine splenocytes leads tosqstm1/p62 mediated nrf2-keap1 antioxidant pathway activation and inducedproteasome-mediated degradation in malaria | Sengupta A, Mukherjee S, ..., Bhattacharyya A | 10.1016/j.micpath.2020.104289 |