Product Description: V-type proton ATPase catalytic subunit A
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.3.14 (Transferred entry: 7.1.2.2);7.1.2.2 (H(+)-transporting two-sector ATPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020020 | food vacuole |
| Component | GO:0005886 | plasma membrane |
| Component | GO:0005774 | vacuolar membrane |
| Component | GO:0000221 | vacuolar proton-transporting V-type ATPase, V1 domain |
| Function | GO:0046961 | proton-transporting ATPase activity, rotational mechanism |
| Process | GO:1902600 | proton transmembrane transport |
| Process | GO:0007035 | vacuolar acidification |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.22 | 0.89 |
| Trophozoite | expressed | 0.50 | 0.70 |
| Schizont | expressed | 0.29 | 0.80 |
| Gametocyte | expressed | 0.35 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1311900
Resistome Missense Mutations: Q225K, R353K
Resistome Compounds with Missense Mutations: SQ109
Resistome # Samples with Disruptive Mutations: 4 (4 missense, 4 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.93 | MFS: -2.245 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1410400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q76NM6
"Best" AlphaFill ligand hit: QUE (quercetin, Local RMSD=0.02) with 2JJ2 (Global RMSD=4.72)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100902
Most Similar Human Ortholog: C9JA17
TM-align score: 0.99 | RMSD: 0.59
Seq Identity: 0.54 | Length: 184 / 611
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000114573 | ATPase H+ transporting V1 subunit A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 42 | 28 | 52 |
| disruptive | 1 | 9 | 6 | 32 |
| missense | 1 | 9 | 6 | 26 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 112 | 34 | 28 |
| disruptive | 2 | 50 | 44 | 130 |
| missense | 1 | 35 | 30 | 79 |
PlasmoDB Total SNPs: 119
Non-coding: 103 | Synonymous: 12 | Nonsynonymous: 2 | Stop Codon: 2
Protein Length: 611 | Molecular Weight (kDa): 68.576
PDB ID(s): None
Isoelectric Point: 5.36
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000194 | ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain |
| InterPro | IPR004100 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain |
| InterPro | IPR005725 | ATPase, V1 complex, subunit A |
| InterPro | IPR020003 | ATPase, alpha/beta subunit, nucleotide-binding domain, active site |
| InterPro | IPR022878 | V-type ATP synthase catalytic alpha chain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR031686 | ATPsynthase alpha/beta subunit, N-terminal extension |
| InterPro | IPR036121 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily |
| PFam | PF00006 | ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain |
| PFam | PF02874 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain |
| PFam | PF16886 | ATPsynthase alpha/beta subunit, N-terminal extension |
| Superfamily | SSF47917 | ATPase, F1/V1/A1 complex, alpha/beta subunit, N-terminal domain superfamily |
| Superfamily | SSF50615 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF52540 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 10915784 | Vacuolar H(+)-ATPase localized in plasma membranes of malaria parasite cells,Plasmodium falciparum, is involved in regional acidification of parasitizederythrocytes. | Hayashi M, Yamada H, ..., Moriyama Y | 10.1074/jbc.M003323200 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 17653272 | Rapid identification of malaria vaccine candidates based on alpha-helical coiledcoil protein motif. | Villard V, Agak GW, ..., Corradin G | 10.1371/journal.pone.0000645 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |