Product Description: translation initiation factor IF-2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0005525 | GTP binding |
| Function | GO:0003924 | GTPase activity |
| Function | GO:0003743 | translation initiation factor activity |
| Process | GO:0006413 | translational initiation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.03 | 0.98 |
| Trophozoite | not expressed | 0.19 | 0.88 |
| Schizont | not expressed | 0.04 | 0.97 |
| Gametocyte | possibly expressed | 0.24 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1312400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.148 | MFS: -2.794 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1410900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEJ7
"Best" AlphaFill ligand hit: PPV (pyrophosphate, Local RMSD=0.25) with 2PMD (Global RMSD=7.34)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 63 | 33 | 75 |
| disruptive | 25 | 114 | 74 | 179 |
| missense | 21 | 106 | 71 | 160 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 117 | 54 | 78 |
| disruptive | 53 | 252 | 126 | 272 |
| missense | 40 | 224 | 111 | 200 |
PlasmoDB Total SNPs: 97
Non-coding: 7 | Synonymous: 54 | Nonsynonymous: 35 | Stop Codon: 1
Protein Length: 1128 | Molecular Weight (kDa): 132.291
UniProt ID(s): Q8IEJ7
PDB ID(s): None
Isoelectric Point: 6.99
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000795 | Transcription factor, GTP-binding domain |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR009000 | Translation protein, beta-barrel domain superfamily |
| InterPro | IPR015760 | Translation initiation factor IF- 2 |
| InterPro | IPR023115 | Translation initiation factor IF- 2, domain 3 |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR036925 | Translation initiation factor IF-2, domain 3 superfamily |
| PFam | PF00009 | Transcription factor, GTP-binding domain |
| PFam | PF11987 | Translation initiation factor IF- 2, domain 3 |
| Superfamily | SSF50447 | Translation protein, beta-barrel domain superfamily |
| Superfamily | SSF52156 | Translation initiation factor IF-2, domain 3 superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 25689481 | Targeting and function of proteins mediating translation initiation in organellesof Plasmodium falciparum. | Haider A, Allen SM, ..., Habib S | 10.1111/mmi.12972 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 16877317 | The archaeal origins of the eukaryotic translational system | Hartman H, Favaretto P, Smith TF | 10.1155/2006/431618 |
| 6337147 | Initiation factor and ribosome levels are coordinately controlled in Escherichiacoli growing at different rates | Howe JG, Hershey JW | https://pubmed.ncbi.nlm.nih.gov/6337147/ |