Product Description: 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.2.4.4 (3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005947 | mitochondrial alpha-ketoglutarate dehydrogenase complex |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0003826 | alpha-ketoacid dehydrogenase activity |
| Function | GO:0003729 | mRNA binding |
| Process | GO:0009083 | branched-chain amino acid catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.06 | 0.97 |
| Trophozoite | expressed | 0.32 | 0.81 |
| Schizont | expressed | 0.41 | 0.74 |
| Gametocyte | expressed | 0.52 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1312600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.204 | MFS: -2.736 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1411100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1411100)
Modification: Disrupted | RMgm-3794
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEJ6
"Best" AlphaFill ligand hit: LMR (l-malate, Local RMSD=0.20) with 6YAK (Global RMSD=2.60)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.2.4.4 | 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) | ATPNADHpyruvateClofibratemicroRNA 29amicroRNA 29b2-oxoglutarate2-oxobutanoate2-oxopentanoateisobutanoyl-CoAisopentanoyl-CoAcinnamylpyruvate... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101723
Most Similar Human Ortholog: F5H5P2
TM-align score: 0.91 | RMSD: 2.06
Seq Identity: 0.39 | Length: 413 / 429
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000248098 | branched chain keto acid dehydrogenase E1 subunit alpha |
| ENSG00000255730 | novel protein |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 14 | 12 | 30 |
| disruptive | 5 | 25 | 17 | 41 |
| missense | 5 | 24 | 15 | 40 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 40 | 16 | 30 |
| disruptive | 7 | 59 | 37 | 77 |
| missense | 7 | 51 | 33 | 55 |
PlasmoDB Total SNPs: 74
Non-coding: 55 | Synonymous: 7 | Nonsynonymous: 12 | Stop Codon: 0
Protein Length: 429 | Molecular Weight (kDa): 49.871
UniProt ID(s): Q8IEJ6
PDB ID(s): None
Isoelectric Point: 7.46
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001017 | Dehydrogenase, E1 component |
| InterPro | IPR029061 | Thiamin diphosphate-binding fold |
| InterPro | IPR034616 | 2-oxoisovalerate dehydrogenase subunit alpha |
| PFam | PF00676 | Dehydrogenase, E1 component |
| Superfamily | SSF52518 | Thiamin diphosphate-binding fold |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |