PF3D7_1313000 (NEDD8)

Genome location: Pf3D7_13_v3:553,067..554,779(-)

Genome classification: Core

Function and Localization

Product Description: ubiquitin-like protein Nedd8

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Function	GO:0005515	protein binding
Function	GO:0031386	protein tag
Function	GO:0031625	ubiquitin protein ligase binding
Process	GO:0019941	modification-dependent protein catabolic process
Process	GO:0045116	protein neddylation
Process	GO:0030162	regulation of proteolysis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.03	0.99
Trophozoite	expressed	0.22	0.86
Schizont	expressed	0.15	0.89
Gametocyte	expressed	0.17	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1313000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.472 | MFS: -3.759 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEI4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q15843	NEDD8		OMA, OrthoDB	Pevonedistat

Orthology Information

Ortholog Group (OrthoMCL): OG6_121187

No human ortholog(s)

Protein Information

Protein Length: 76 | Molecular Weight (kDa): 8.684

UniProt ID(s): Q8IEI4

PDB ID(s): None

Isoelectric Point: 10.1

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000626	Ubiquitin-like domain
InterPro	IPR019956	Ubiquitin domain
InterPro	IPR029071	Ubiquitin-like domain superfamily
InterPro	IPR038738	Nedd8-like ubiquitin
PFam	PF00240	Ubiquitin-like domain
Superfamily	SSF54236	Ubiquitin-like domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	1	0	0
disruptive	0	0	0	0
missense	0	0	0	0

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	0	1	0	0
disruptive	0	0	0	0
missense	0	0	0	0

PlasmoDB Total SNPs: 109

Non-coding: 104 | Synonymous: 5 | Nonsynonymous: 0 | Stop Codon: 0

PMID	Title	Authors	DOI/Link
33214620	Characterization of Plasmodium falciparum NEDD8 and identification of cullins asits substrates.	Bhattacharjee M, Adhikari N, ..., Sijwali PS	10.1038/s41598-020-77001-5
38411954	Neddylation is essential for malaria transmission in Plasmodium berghei	Nayak B, Paul P, Mishra S	10.1128/mbio.00232-24