PF3D7_1313200 (MTFMT)

Genome location: Pf3D7_13_v3:565,649..568,253(+)

Genome classification: Core

Function and Localization

Product Description: methionyl-tRNA formyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.2.9 (Methionyl-tRNA formyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005739	mitochondrion
Function	GO:0004479	methionyl-tRNA formyltransferase activity
Process	GO:0071951	conversion of methionyl-tRNA to N-formyl-methionyl-tRNA
Process	GO:0006413	translational initiation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.02	0.99
Trophozoite	no data	0.13	0.91
Schizont	no data	0.03	0.98
Gametocyte	no data	0.14	0.91

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1313200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.461 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEI1

"Best" AlphaFill ligand hit: MPO (3[n-morpholino]propane sulfonic acid, Local RMSD=0.52) with 4LXQ (Global RMSD=8.18)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.2.9	methionyl-tRNA formyltransferase	tRNAfMetSB-734453aminopterinmethotrexatetetrahydrofolatereduced aminopterin10-Formylaminopterin5-methyltetrahydrofolate5-formyltetrahydrofolate10-formylpteroylglutamic acid5,10-methylenetetrahydrofolate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101497

Most Similar Human Ortholog: Q96DP5

TM-align score: 0.66 | RMSD: 3.64

Seq Identity: 0.16 | Length: 300 / 665

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000103707	mitochondrial methionyl-tRNA formyltransferase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	33	26	51
disruptive	11	71	42	129
missense	10	70	42	121

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	64	37	51
disruptive	21	173	81	186
missense	18	153	72	141

PlasmoDB Total SNPs: 120

Non-coding: 51 | Synonymous: 44 | Nonsynonymous: 25 | Stop Codon: 0

Protein Information

Protein Length: 665 | Molecular Weight (kDa): 79.643

UniProt ID(s): Q8IEI1

PDB ID(s): None

Isoelectric Point: 9.82

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002376	Formyl transferase, N-terminal
InterPro	IPR005793	Formyl transferase, C-terminal
InterPro	IPR011034	Formyl transferase-like, C-terminal domain superfamily
InterPro	IPR036477	Formyl transferase, N-terminal domain superfamily
PFam	PF00551	Formyl transferase, N-terminal
PFam	PF02911	Formyl transferase, C-terminal
Superfamily	SSF50486	Formyl transferase-like, C-terminal domain superfamily
Superfamily	SSF53328	Formyl transferase, N-terminal domain superfamily