PF3D7_1313400

Genome location: Pf3D7_13_v3:569,563..572,777(-)

Genome classification: Core

Function and Localization

Product Description: DEAD box helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 3.6.1.3 (Deleted entry)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0010494	cytoplasmic stress granule
Component	GO:0005634	nucleus
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity
Function	GO:0004386	helicase activity
Process	GO:0016973	poly(A)+ mRNA export from nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	possibly expressed	0.12	0.94
Trophozoite	expressed	0.75	0.59
Schizont	expressed	0.40	0.74
Gametocyte	expressed	0.75	0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1313400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.913 | MFS: -2.55 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1411800)

Relative Growth Rate: 0.07 ± 0.18
Confidence: 4.87

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEH9

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=1.24) with 4D26 (Global RMSD=10.76)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.1.3	adenosinetriphosphatase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_133913

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	43	29	49
disruptive	19	94	61	152
missense	15	91	53	135

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	85	47	61
disruptive	35	228	125	261
missense	24	194	102	191

PlasmoDB Total SNPs: 95

Non-coding: 49 | Synonymous: 25 | Nonsynonymous: 21 | Stop Codon: 0

Protein Information

Protein Length: 830 | Molecular Weight (kDa): 99.269

UniProt ID(s): Q8IEH9

PDB ID(s): None

Isoelectric Point: 9.34

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase