PF3D7_1313700

Genome location: Pf3D7_13_v3:586,557..587,858(-)

Genome classification: Core

Function and Localization

Product Description: Maf-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.1.9 (Nucleotide diphosphatase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0047429	nucleoside-triphosphate diphosphatase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.98
Trophozoite	not expressed	0.13	0.91
Schizont	not expressed	0.03	0.98
Gametocyte	possibly expressed	0.12	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1313700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.703 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEH6

"Best" AlphaFill ligand hit: DUT (deoxyuridine-5'-triphosphate, Local RMSD=0.26) with 1EXC (Global RMSD=1.36)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.1.9	nucleotide diphosphatase	ATPADPAMPGDPCTPUTPSDSUMPGSHGMPITPNaF...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101328

Most Similar Human Ortholog: O95671

TM-align score: 0.74 | RMSD: 1.95

Seq Identity: 0.38 | Length: 209 / 264

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000169093	acetylserotonin O-methyltransferase like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	9	17	21
disruptive	7	37	23	63
missense	7	37	23	60

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	28	14	24
disruptive	13	81	36	66
missense	11	80	32	57

PlasmoDB Total SNPs: 88

Non-coding: 72 | Synonymous: 9 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 264 | Molecular Weight (kDa): 30.554

UniProt ID(s): Q8IEH6

PDB ID(s): None

Isoelectric Point: 6.24

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003697	Nucleoside triphosphate pyrophosphatase Maf-like protein
InterPro	IPR029001	Inosine triphosphate pyrophosphatase-like
PFam	PF02545	Nucleoside triphosphate pyrophosphatase Maf-like protein
Superfamily	SSF52972	Inosine triphosphate pyrophosphatase-like