PF3D7_1314200 (TERT)

Genome location: Pf3D7_13_v3:611,085..618,888(+)

Genome classification: Core

Function and Localization

Product Description: telomerase reverse transcriptase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.49 (RNA-directed DNA polymerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Component	GO:0000333	telomerase catalytic core complex
Function	GO:0070034	telomerase RNA binding
Function	GO:0003721	telomerase RNA reverse transcriptase activity
Function	GO:0042162	telomeric DNA binding
Process	GO:0032203	telomere formation via telomerase
Process	GO:0007004	telomere maintenance via telomerase

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.21	0.90
Trophozoite	possibly expressed	0.90	0.55
Schizont	possibly expressed	0.18	0.90
Gametocyte	possibly expressed	0.53	0.72

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1314200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.995 | MFS: -2.967 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1412600)

Modification: Disrupted | RMgm-1136

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEH2

"Best" AlphaFill ligand hit: MPO (3[n-morpholino]propane sulfonic acid, Local RMSD=4.36) with 4LXY (Global RMSD=29.11)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.49	RNA-directed DNA polymerase	BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q673L6	Telomerase reverse transcriptase	Rattus norvegicus	OrthoDB, OrthoMCL BLAST	CHEMBL3109741
O14746	Telomerase reverse transcriptase	Homo sapiens	OrthoDB	CHEMBL94007CHEMBL94007Ethidium bromideCHEMBL94007...

Orthology Information

Ortholog Group (OrthoMCL): OG6_532513

No human ortholog(s)

Protein Information

Protein Length: 2518 | Molecular Weight (kDa): 304.138

UniProt ID(s): Q8IEH2

PDB ID(s): None

Isoelectric Point: 10.58

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003545	Telomerase reverse transcriptase
InterPro	IPR021891	Telomerase ribonucleoprotein complex - RNA-binding domain
PFam	PF12009	Telomerase ribonucleoprotein complex - RNA-binding domain
Superfamily	N/A	N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	19	125	89	203
disruptive	55	334	178	465
missense	40	299	165	415

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	40	260	129	211
disruptive	164	667	321	640
missense	88	569	277	476

PlasmoDB Total SNPs: 225

Non-coding: 47 | Synonymous: 120 | Nonsynonymous: 54 | Stop Codon: 4

PMID	Title	Authors	DOI/Link
9447988	Plasmodium falciparum telomerase: de novo telomere addition to telomeric andnontelomeric sequences and role in chromosome healing.	Bottius E, Bakhsis N, Scherf A	10.1128/MCB.18.2.919
15722485	The unusually large Plasmodium telomerase reverse-transcriptase localizes in adiscrete compartment associated with the nucleolus.	Figueiredo LM, Rocha EP, ..., Scherf A	10.1093/nar/gki260