PF3D7_1314900 (P44)

Genome location: Pf3D7_13_v3:632,005..635,048(+)

Genome classification: Core

Function and Localization

Product Description: general transcription factor IIH subunit 2

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Component	GO:0005675	transcription factor TFIIH holo complex
Function	GO:0005515	protein binding
Process	GO:0006289	nucleotide-excision repair
Process	GO:0006357	regulation of transcription by RNA polymerase II

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.27	0.87
Trophozoite	expressed	0.43	0.74
Schizont	expressed	0.05	0.96
Gametocyte	not expressed	0.25	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1314900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.113 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1413400)

Relative Growth Rate: 0.04 ± 0.08
Confidence: 6.46

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEG6

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102284

Most Similar Human Ortholog: Q6P1K8

TM-align score: 0.90 | RMSD: 1.97

Seq Identity: 0.27 | Length: 373 / 401

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000145736	general transcription factor IIH subunit 2
ENSG00000183474	GTF2H2 family member C
ENSG00000274675	GTF2H2 family member C, copy 2
ENSG00000275045	general transcription factor IIH subunit 2
ENSG00000276910	general transcription factor IIH subunit 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	22	14	22
disruptive	2	26	9	35
missense	2	26	9	33

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	41	15	31
disruptive	4	49	25	84
missense	4	43	21	60

PlasmoDB Total SNPs: 166

Non-coding: 157 | Synonymous: 5 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 401 | Molecular Weight (kDa): 45.986

UniProt ID(s): M9VUH5, Q8IEG6

PDB ID(s): None

Isoelectric Point: 6.54

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002035	von Willebrand factor, type A
InterPro	IPR004595	TFIIH C1-like domain
InterPro	IPR007198	Ssl1-like
InterPro	IPR012170	TFIIH subunit Ssl1/p44
InterPro	IPR036465	von Willebrand factor A-like domain superfamily
PFam	PF04056	Ssl1-like
PFam	PF07975	TFIIH C1-like domain
Superfamily	SSF53300	von Willebrand factor A-like domain superfamily
Superfamily	SSF57889