PF3D7_1315600 (PIS)

Genome location: Pf3D7_13_v3:654,882..657,517(-)

Genome classification: Core

Function and Localization

Product Description: CDP-diacylglycerol--inositol 3-phosphatidyltransferase

SignalP Peptide: N/A

# Transmembrane Domains: 3

EC Numbers: 2.7.8.11 (CDP-diacylglycerol--inositol 3-phosphatidyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005794	Golgi apparatus
Function	GO:0003881	CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity
Process	GO:0006661	phosphatidylinositol biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.03	0.98
Trophozoite	expressed	0.29	0.82
Schizont	expressed	0.22	0.84
Gametocyte	expressed	0.21	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1315600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.126 | MFS: -3.21 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5B7

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.8.11	CDP-diacylglycerol-inositol 3-phosphatidyltransferase	ATPADPUDPAMPGTPCTPUTPCDPTTPCaCl2ZnCl2Tween 80...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102569

Most Similar Human Ortholog: O14735

TM-align score: 0.90 | RMSD: 1.84

Seq Identity: 0.27 | Length: 201 / 209

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000103502	CDP-diacylglycerol--inositol 3-phosphatidyltransferase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	11	2	14
disruptive	3	13	6	21
missense	3	13	6	16

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	17	8	19
disruptive	8	30	19	51
missense	4	24	18	42

PlasmoDB Total SNPs: 201

Non-coding: 178 | Synonymous: 17 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 209 | Molecular Weight (kDa): 23.925

UniProt ID(s): C0H5B7, Q2MJB9

PDB ID(s): None

Isoelectric Point: 9.75

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000462	CDP-alcohol phosphatidyltransferase
InterPro	IPR014387	CDP-diacylglycerol-inositol 3-phosphatidyltransferase, eukaryote
PFam	PF01066	CDP-alcohol phosphatidyltransferase
Superfamily	N/A	N/A