Product Description: tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.220 (tRNA (adenine(58)-N(1))-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0031515 | tRNA (m1A) methyltransferase complex |
| Process | GO:0030488 | tRNA methylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.20 | 0.90 |
| Trophozoite | expressed | 0.34 | 0.80 |
| Schizont | expressed | 0.07 | 0.95 |
| Gametocyte | expressed | 0.21 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1315700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.134 | MFS: -3.057 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEF7
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.52) with 5CCB (Global RMSD=1.58)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.220 | tRNA (adenine58-N1)-methyltransferase |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100880
Most Similar Human Ortholog: Q96FX7
TM-align score: 0.86 | RMSD: 2.20
Seq Identity: 0.30 | Length: 266 / 316
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000166166 | tRNA methyltransferase 61A |
| ENSG00000171103 | tRNA methyltransferase 61B |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 15 | 9 | 18 |
| disruptive | 8 | 41 | 18 | 40 |
| missense | 8 | 41 | 17 | 34 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 28 | 18 | 20 |
| disruptive | 20 | 78 | 33 | 78 |
| missense | 18 | 70 | 28 | 52 |
PlasmoDB Total SNPs: 155
Non-coding: 132 | Synonymous: 12 | Nonsynonymous: 11 | Stop Codon: 0
Protein Length: 316 | Molecular Weight (kDa): 37.204
UniProt ID(s): Q8IEF7
PDB ID(s): None
Isoelectric Point: 5.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR014816 | tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14 |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF08704 | tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14 |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 10779558 | The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine)methyltransferase of Saccharomyces cerevisiae | Anderson J, Phan L, Hinnebusch AG | 10.1073/pnas.090102597 |
| 10330157 | GCD14p, a repressor of GCN4 translation, cooperates with Gcd10p and Lhp1p in thematuration of initiator methionyl-tRNA in Saccharomyces cerevisiae | Calvo O, Cuesta R, ..., Tamame M | 10.1128/MCB.19.6.4167 |
| 16043508 | The bipartite structure of the tRNA m1A58 methyltransferase from S. cerevisiae is conserved in humans | Ozanick S, Krecic A, Andersland J, Anderson JT | 10.1261/rna.5040605 |
| 17932071 | Conserved amino acids in each subunit of the heteroligomeric tRNA m1A58 Mtasefrom Saccharomyces cerevisiae contribute to tRNA binding | Ozanick SG, Bujnicki JM, Sem DS, Anderson JT | 10.1093/nar/gkm574 |
| 22102254 | Crystallization and preliminary X-ray diffraction crystallographic study of tRNAm(1)A58 methyltransferase from Saccharomyces cerevisiae | Qiu X, Huang K, Ma J, Gao Y | 10.1107/S174430911103733X |
| 9539420 | Identification of GCD14 and GCD15, novel genes required for translationalrepression of GCN4 mRNA in Saccharomyces cerevisiae | Cuesta R, Hinnebusch AG, Tamame M | 10.1093/genetics/148.3.1007 |
| 15302822 | Role of a 300-kilodalton nuclear complex in the maturation of Trypanosoma bruceiinitiator methionyl-tRNA | Arhin GK, Shen S, ..., Tschudi C | 10.1128/EC.3.4.893-899.2004 |
| 27582183 | Crystal structure of the two-subunit tRNA m(1)A58 methyltransferase TRM6-TRM61from Saccharomyces cerevisiae | Wang M, Zhu Y, ..., Li X | 10.1038/srep32562 |
| 8948101 | Sequencing analysis of a 40.2 kb fragment of yeast chromosome X reveals 19 openreading frames including URA2 (5' end), TRK1, PBS2, SPT10, GCD14, RPE1, PHO86,NCA3, ASF1, CCT7, GZF3, two tRNA genes, three remnant delta elements and a Ty4transposon | Cziepluch C, Kordes E, Pujol A, Jauniaux JC | 10.1002/(SICI)1097-0061(199611)12:14<1471::AID-YEA30>3.0.CO;2-4 |