PF3D7_1316200

Genome location: Pf3D7_13_v3:679,376..681,341(+)

Genome classification: Core

Function and Localization

Product Description: ADP-ribosylation factor, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0005525	GTP binding
Function	GO:0003924	GTPase activity
Process	GO:0006886	intracellular protein transport
Process	GO:0016192	vesicle-mediated transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.05	0.97
Trophozoite	not expressed	0.03	0.98
Schizont	expressed	0.14	0.91
Gametocyte	not expressed	0.03	0.98

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1316200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -2.95 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1414700)

Relative Growth Rate: 0.96 ± 0.05
Confidence: 7.51

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1414700)

Modification: Disrupted | RMgm-3812

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEF2

"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=0.49) with 1Z6X (Global RMSD=4.48)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532813

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	16	8	11
disruptive	6	12	6	21
missense	6	12	6	16

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	24	10	13
disruptive	10	29	15	47
missense	7	22	12	32

PlasmoDB Total SNPs: 104

Non-coding: 89 | Synonymous: 11 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 224 | Molecular Weight (kDa): 26.563

UniProt ID(s): Q8IEF2

PDB ID(s): None

Isoelectric Point: 8.2

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006689	Small GTPase superfamily, ARF/SAR type
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00025	Small GTPase superfamily, ARF/SAR type
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase