Product Description: choline-phosphate cytidylyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.15 (Choline-phosphate cytidylyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004105 | choline-phosphate cytidylyltransferase activity |
| Function | GO:0042802 | identical protein binding |
| Function | GO:0031210 | phosphatidylcholine binding |
| Process | GO:0006656 | phosphatidylcholine biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.39 | 0.81 |
| Trophozoite | expressed | 1.28 | 0.38 |
| Schizont | expressed | 1.67 | 0.29 |
| Gametocyte | expressed | 0.61 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1316600
Resistome Missense Mutations: D238E
Resistome Compounds with Missense Mutations: MMV009108
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.854 | MFS: -2.86 | #Insertions: 1
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1415100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1415100)
Modification: Tagged | RMgm-339
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEE9
"Best" AlphaFill ligand hit: HV2 (1,1-bis(oxidanylidene)thietan-3-ol, Local RMSD=0.17) with 7PVF (Global RMSD=2.73)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.15 | choline-phosphate cytidylyltransferase | ATPcalpainceramideBlue H-BphosphateDibucaineBlue MX-RpropanololCDPcholineTetracaineGreen H-4Gsphingosine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101770
Most Similar Human Ortholog: H7C1T3
TM-align score: 0.74 | RMSD: 2.65
Seq Identity: 0.44 | Length: 167 / 896
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000102230 | phosphate cytidylyltransferase 1, choline, beta |
| ENSG00000161217 | phosphate cytidylyltransferase 1, choline, alpha |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 39 | 22 | 47 |
| disruptive | 22 | 55 | 38 | 83 |
| missense | 16 | 45 | 26 | 67 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 77 | 29 | 41 |
| disruptive | 43 | 122 | 57 | 144 |
| missense | 22 | 88 | 48 | 93 |
PlasmoDB Total SNPs: 197
Non-coding: 113 | Synonymous: 59 | Nonsynonymous: 25 | Stop Codon: 0
Protein Length: 896 | Molecular Weight (kDa): 104.782
PDB ID(s): 4ZCP, 4ZCQ, 4ZCR, 4ZCS, 4ZCT
Isoelectric Point: 6.59
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004821 | Cytidyltransferase-like domain |
| InterPro | IPR041723 | CTP:phosphocholine cytidylyltransferase domain |
| PFam | PF01467 | Cytidyltransferase-like domain |
| Superfamily | SSF52374 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 21705805 | PG12, a phospholipid analog with potent antimalarial activity, inhibitsPlasmodium falciparum CTP:phosphocholine cytidylyltransferase activity. | Gonzalez-Bulnes P, Bobenchik AM, ..., Ben Mamoun C | 10.1074/jbc.M111.268946 |
| 23578277 | Evolutionary and mechanistic insights into substrate and product accommodation ofCTP:phosphocholine cytidylyltransferase from Plasmodium falciparum. | Nagy GN, Marton L, ..., Vertessy BG | 10.1111/febs.12282 |
| 25771858 | Plasmodium falciparum CTP:phosphocholine cytidylyltransferase possesses twofunctional catalytic domains and is inhibited by a CDP-choline analog selectedfrom a virtual screening. | Contet A, Pihan E, ..., Cerdan R | 10.1016/j.febslet.2015.03.003 |
| 26083347 | Molecular Mechanism for the Thermo-Sensitive Phenotype of CHO-MT58 Cell LineHarbouring a Mutant CTP:Phosphocholine Cytidylyltransferase. | Marton L, Nagy GN, ..., Vertessy BG | 10.1371/journal.pone.0129632 |
| 33184408 | Identification of a nuclear localization signal in the Plasmodium falciparum CTP:phosphocholine cytidylyltransferase enzyme. | Izrael R, Marton L, ..., Vertessy BG | 10.1038/s41598-020-76829-1 |
| 38433581 | Relationships between functional alpha and beta diversities of flea parasites andtheir small mammalian hosts | Krasnov BR, Khokhlova IS, ..., van der Mescht L | 10.1017/S0031182024000283 |