Product Description: glycerol-3-phosphate 1-O-acyltransferase
SignalP Peptide: MIKFSVLSFLTTLFLVVLKFHVVKN
# Transmembrane Domains: 0
EC Numbers: 2.3.1.15 (Glycerol-3-phosphate 1-O-acyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0020011 | apicoplast |
| Function | GO:0004366 | glycerol-3-phosphate O-acyltransferase activity |
| Process | GO:0045017 | glycerolipid biosynthetic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.01 | 1.00 |
| Trophozoite | possibly expressed | 0.04 | 0.98 |
| Schizont | not expressed | 0.03 | 0.98 |
| Gametocyte | possibly expressed | 0.05 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1318200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.463 | #Insertions: 3
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1416700)
Modification: Tagged | RMgm-1469
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IED4
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.15 | glycerol-3-phosphate 1-O-acyltransferase | NEMEDTAMgCl2CaCl2oleatepapainacetonecholatePronaseTrypsinspermineacyl-CoA... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 13 | 7 | 35 |
| disruptive | 4 | 30 | 18 | 48 |
| missense | 4 | 29 | 17 | 47 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 23 | 25 | 31 |
| disruptive | 11 | 76 | 32 | 78 |
| missense | 10 | 65 | 29 | 59 |
PlasmoDB Total SNPs: 20
Non-coding: 0 | Synonymous: 8 | Nonsynonymous: 12 | Stop Codon: 0
Protein Length: 400 | Molecular Weight (kDa): 47.12
UniProt ID(s): Q8IED4
PDB ID(s): None
Isoelectric Point: 9.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002123 | Phospholipid/glycerol acyltransferase |
| InterPro | IPR016222 | Glycerol-3-phosphate O-acyltransferase, chloroplast |
| PFam | PF01553 | Phospholipid/glycerol acyltransferase |
| Superfamily | SSF69593 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 27324409 | Characterization of the Plasmodium falciparum and P. berghei glycerol 3-phosphateacyltransferase involved in F ASII fatty acid utilization in the malaria parasiteapicoplast | Shears MJ, MacRae JI, ..., McFadden GI | 10.1111/cmi.12633 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |