Product Description: translocation protein SEC63, putative
SignalP Peptide: N/A
# Transmembrane Domains: 3
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031207 | Sec62/Sec63 complex |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0008320 | protein transmembrane transporter activity |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0006614 | SRP-dependent cotranslational protein targeting to membrane |
| Process | GO:0006620 | posttranslational protein targeting to endoplasmic reticulum membrane |
| Process | GO:0031204 | posttranslational protein targeting to membrane, translocation |
| Process | GO:0006457 | protein folding |
| Process | GO:0045047 | protein targeting to ER |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.64 | 0.71 |
| Trophozoite | expressed | 0.82 | 0.55 |
| Schizont | expressed | 0.56 | 0.66 |
| Gametocyte | expressed | 0.35 | 0.79 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1318800
Resistome Missense Mutations: R284G
Resistome Compounds with Missense Mutations: TCMDC-124553/MMV019869
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.154 | MFS: -2.828 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1417300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEC8
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102234
Most Similar Human Ortholog: Q9UGP8
TM-align score: 0.69 | RMSD: 4.84
Seq Identity: 0.18 | Length: 540 / 651
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000025796 | SEC63 homolog, protein translocation regulator |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 50 | 14 | 34 |
| disruptive | 1 | 16 | 20 | 44 |
| missense | 1 | 16 | 15 | 25 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 72 | 26 | 27 |
| disruptive | 6 | 69 | 51 | 113 |
| missense | 4 | 40 | 39 | 62 |
PlasmoDB Total SNPs: 294
Non-coding: 254 | Synonymous: 32 | Nonsynonymous: 8 | Stop Codon: 0
Protein Length: 651 | Molecular Weight (kDa): 75.872
UniProt ID(s): Q8IEC8
PDB ID(s): None
Isoelectric Point: 6.67
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001623 | DnaJ domain |
| InterPro | IPR004179 | Sec63 domain |
| InterPro | IPR014756 | Immunoglobulin E-set |
| InterPro | IPR018253 | DnaJ domain, conserved site |
| InterPro | IPR027137 | Translocation protein Sec63 |
| InterPro | IPR036869 | Chaperone J-domain superfamily |
| PFam | PF00226 | DnaJ domain |
| PFam | PF02889 | Sec63 domain |
| Superfamily | SSF158702 | Chaperone J-domain superfamily |
| Superfamily | SSF46565 | Immunoglobulin E-set |
| Superfamily | SSF81296 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 30127496 | Plasmepsin V cleaves malaria effector proteins in a distinct endoplasmicreticulum translocation interactome for export to the erythrocyte. | Marapana DS, Dagley LF, ..., Cowman AF | 10.1038/s41564-018-0219-2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 36116515 | Effect of Sec62 on the conformation of the Sec61 channel in yeast | Bhadra P, Romisch K, Helms V | 10.1016/j.bbamem.2022.184050 |
| 34492269 | Rapid inactivation of the yeast Sec complex selectively blocks transport ofpost-translationally translocated proteins | Yi JK, Fujita H, Mandon EC, Gilmore R | 10.1016/j.jbc.2021.101171 |