PF3D7_1319100

Genome location: Pf3D7_13_v3:789,267..792,352(+)

Genome classification: Core

Function and Localization

Product Description: ubiquitin carboxyl-terminal hydrolase MINDY, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.19.12 (Ubiquitinyl hydrolase 1)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.95	0.28
Trophozoite	expressed	0.90	0.53
Schizont	expressed	0.70	0.60
Gametocyte	expressed	0.99	0.48

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1319100

Resistome Missense Mutations: N792K

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.371 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K970

"Best" AlphaFill ligand hit: AYE (allylamine, Local RMSD=1.37) with 5JQS (Global RMSD=1.48)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.19.12	ubiquitinyl hydrolase 1	KClNEMDTTEDTAEGTARNAisiRNApeptidesAntibodyubiquitinLDN-574442-propenal...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102202

Most Similar Human Ortholog: Q8N5J2

TM-align score: 0.54 | RMSD: 2.38

Seq Identity: 0.33 | Length: 270 / 803

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000128923	MINDY lysine 48 deubiquitinase 2
ENSG00000143409	MINDY lysine 48 deubiquitinase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	16	52	19	54
disruptive	29	103	55	123
missense	11	59	36	83

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	23	101	38	48
disruptive	63	216	119	297
missense	26	119	87	158

PlasmoDB Total SNPs: 258

Non-coding: 113 | Synonymous: 113 | Nonsynonymous: 32 | Stop Codon: 0

Protein Information

Protein Length: 803 | Molecular Weight (kDa): 94.813

UniProt ID(s): A0A5K1K970

PDB ID(s): None

Isoelectric Point: 8.22

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007518	MINDY deubiquitinase
InterPro	IPR033979	MINDY deubiquitinase domain
PFam	PF04424	MINDY deubiquitinase domain
Superfamily	N/A	N/A