Product Description: tRNA (guanine(26)-N(2))-dimethyltransferase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.216 (tRNA (guanine(26)-N(2))-dimethyltransferase);2.1.1.32 (Transferred entry: 2.1.1.213, 2.1.1.214, 2.1.1.215 and 2.1.1.216)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0004809 | tRNA (guanine-N2-)-methyltransferase activity |
| Process | GO:0002940 | tRNA N2-guanine methylation |
| Process | GO:0030488 | tRNA methylation |
| Process | GO:0006400 | tRNA modification |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.04 | 0.53 |
| Trophozoite | expressed | 0.76 | 0.59 |
| Schizont | expressed | 0.19 | 0.89 |
| Gametocyte | possibly expressed | 0.48 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1319300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -3.02 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1417800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1417800)
Modification: Disrupted | RMgm-3822
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEC3
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.75) with 2EJU (Global RMSD=4.26)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101071
Most Similar Human Ortholog: K7EJX9
TM-align score: 0.93 | RMSD: 1.61
Seq Identity: 0.27 | Length: 190 / 804
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000104907 | tRNA methyltransferase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 54 | 36 | 50 |
| disruptive | 24 | 76 | 44 | 124 |
| missense | 19 | 66 | 39 | 107 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 109 | 38 | 52 |
| disruptive | 42 | 172 | 87 | 233 |
| missense | 32 | 143 | 77 | 157 |
PlasmoDB Total SNPs: 177
Non-coding: 84 | Synonymous: 51 | Nonsynonymous: 42 | Stop Codon: 0
Protein Length: 804 | Molecular Weight (kDa): 94.817
UniProt ID(s): Q8IEC3
PDB ID(s): None
Isoelectric Point: 8.43
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002905 | tRNA methyltransferase, Trm1 |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF02005 | tRNA methyltransferase, Trm1 |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 35517506 | Regulation of Peroxisome Homeostasis by Post-Translational Modification in theMethylotrophic Yeast Komagataella phaffii | Ohsawa S, Oku M, Yurimoto H, Sakai Y | 10.3389/fcell.2022.887806 |
| 22895820 | The yeast rapid tRNA decay pathway competes with elongation factor 1A forsubstrate tRNAs and acts on tRNAs lacking one or more of several modifications | Dewe JM, Whipple JM, ..., Phizicky EM | 10.1261/rna.033654.112 |
| 34462231 | Regulation of alcohol oxidase gene expression in methylotrophic yeast Ogataeaminuta | Yoko-O T, Komatsuzaki A, ..., Chiba Y | 10.1016/j.jbiosc.2021.08.001 |
| 26720005 | RNA Polymerase III Output Is Functionally Linked to tRNA Dimethyl-G26Modification | Arimbasseri AG, Blewett NH, ..., Maraia RJ | 10.1371/journal.pgen.1005671 |
| 22912484 | Yeast Trm7 interacts with distinct proteins for critical modifications of thetRNAPhe anticodon loop | Guy MP, Podyma BM, ..., Phizicky EM | 10.1261/rna.035287.112 |
| 29846136 | Intracellular mechanism by which arsenite activates the yeast stress MAPK Hog1 | Lee J, Levin DE | 10.1091/mbc.E18-03-0185 |