Product Description: ATP-dependent Clp protease adapter protein ClpS
SignalP Peptide: MFKDLKPFFLCIILLLLLIYKCTHS
# Transmembrane Domains: 0
EC Numbers: 3.4.21.92 (Endopeptidase Clp)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0009368 | endopeptidase Clp complex |
| Process | GO:0009657 | plastid organization |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.03 | 0.99 |
| Trophozoite | possibly expressed | 0.42 | 0.75 |
| Schizont | possibly expressed | 0.24 | 0.85 |
| Gametocyte | possibly expressed | 0.15 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1320100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.131 | MFS: -2.856 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1418400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEB2
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.21.92 | Endopeptidase Clp | NEMNeohydrincyclomarinrufomycin ICAANDENYALAAkappa-caseinphosphoarginineCAANDENYALAA-NH2Xaa-Tyr-Leu-Tyr-TrpHigh salt concentrationsdiisopropylfluorophosphatediisopropyl fluorophosphate... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 11 | 4 | 11 |
| disruptive | 1 | 12 | 8 | 14 |
| missense | 1 | 12 | 8 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 26 | 5 | 16 |
| disruptive | 4 | 41 | 11 | 42 |
| missense | 2 | 33 | 9 | 30 |
PlasmoDB Total SNPs: 109
Non-coding: 101 | Synonymous: 5 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 192 | Molecular Weight (kDa): 22.424
UniProt ID(s): Q8IEB2
PDB ID(s): 4O2X
Isoelectric Point: 10.11
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003769 | Adaptor protein ClpS, core |
| InterPro | IPR014719 | Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like |
| PFam | PF02617 | Adaptor protein ClpS, core |
| Superfamily | SSF54736 | Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 26701219 | Structure of a putative ClpS N-end rule adaptor protein from the malaria pathogenPlasmodium falciparum. | AhYoung AP, Koehl A, ..., Egea PF | 10.1002/pro.2868 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 32482878 | Plastid biogenesis in malaria parasites requires the interactions and catalyticactivity of the Clp proteolytic system. | Florentin A, Stephens DR, ..., Muralidharan V | https://pubmed.ncbi.nlm.nih.gov/32482878/ |