PF3D7_1320200

Genome location: Pf3D7_13_v3:836,524..838,735(+)

Genome classification: Core

Function and Localization

Product Description: TBC domain protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0005096	GTPase activator activity
Process	GO:0090630	activation of GTPase activity
Process	GO:0006886	intracellular protein transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.19	0.91
Trophozoite	expressed	0.47	0.72
Schizont	expressed	0.16	0.90
Gametocyte	expressed	0.56	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1320200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.144 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1418500)

Relative Growth Rate: 1.05 ± 0.15
Confidence: 5.19

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1418500)

Modification: Disrupted | RMgm-3827

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEB1

"Best" AlphaFill ligand hit: LAC (lactic acid, Local RMSD=0.57) with 5WBF (Global RMSD=17.43)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102218

Most Similar Human Ortholog: Q9NVG8

TM-align score: 0.79 | RMSD: 3.45

Seq Identity: 0.32 | Length: 352 / 627

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000107021	TBC1 domain family member 13

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	20	24	42
disruptive	11	42	33	84
missense	10	39	32	76

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	72	20	40
disruptive	24	119	65	191
missense	19	100	52	126

PlasmoDB Total SNPs: 84

Non-coding: 52 | Synonymous: 23 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 627 | Molecular Weight (kDa): 74.883

UniProt ID(s): Q8IEB1

PDB ID(s): None

Isoelectric Point: 5.84

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000195	Rab-GTPase-TBC domain
InterPro	IPR035969	Rab-GTPase-TBC domain superfamily
PFam	PF00566	Rab-GTPase-TBC domain
Superfamily	SSF47923	Rab-GTPase-TBC domain superfamily