About Gene List

PF3D7_1320800

Genome location: Pf3D7_13_v3:854,615..856,970(+)

Genome classification: Core

Function and Localization

Product Description: dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.3.1.61 (Dihydrolipoyllysine-residue succinyltransferase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005739 mitochondrion
Function GO:0003723 RNA binding
Function GO:0004149 dihydrolipoyllysine-residue succinyltransferase activity
Process GO:0006103 2-oxoglutarate metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring possibly expressed 0.05 0.97
Trophozoite expressed 0.41 0.75
Schizont expressed 0.08 0.95
Gametocyte expressed 0.31 0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1320800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.398 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1419100)

  • Relative Growth Rate: 1.00 ± 0.11
  • Confidence: 5.85

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1419100)

Modification: Disrupted | RMgm-3829

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IEA6

"Best" AlphaFill ligand hit: CAO (oxidized coenzyme a, Local RMSD=0.15) with 1EAD (Global RMSD=1.40)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.3.1.61 dihydrolipoyllysine-residue succinyltransferase coenzyme Q02-(4-fluorophenyl)-5-(methanesulfonyl)-1,3,4-oxadiazole2-(ethanesulfonyl)-5-(4-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-1,3,4-oxadiazole2-(methanesulfonyl)-5-(4-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-1,3,4-oxadiazole2-(methylsulfonyl)-5-(4-fluoro-3-((prop-2-yn-1-yloxy)methyl)-phenyl)-1,3,4-oxadiazole2-(ethanesulfonyl)-5-(4-fluoro-3-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-1,3,4-oxadiazole2-(4-fluoro-3-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-5-(methanesulfonyl)-1,3,4-oxadiazole2-(2,4-dichloro-3-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-5-(ethanesulfonyl)-1,3,4-oxadiazole2-(2,4-dichloro-3-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-5-(methanesulfonyl)-1,3,4-oxadiazole

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P9WIS7 Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex OMA, OrthoMCL US9073941, 101US9073941, 102US9073941, 28US9073941, 28...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101182

Most Similar Human Ortholog: P36957

TM-align score: 0.75 | RMSD: 3.52

Seq Identity: 0.46 | Length: 357 / 421

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000119689 dihydrolipoamide S-succinyltransferase

Protein Information

Protein Length: 421 | Molecular Weight (kDa): 47.631

UniProt ID(s): Q8IEA6

PDB ID(s): None

Isoelectric Point: 6.74

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000089 Biotin/lipoyl attachment
InterPro IPR001078 2-oxoacid dehydrogenase acyltransferase, catalytic domain
InterPro IPR006255 Dihydrolipoamide succinyltransferase
InterPro IPR011053 Single hybrid motif
PFam PF00198 2-oxoacid dehydrogenase acyltransferase, catalytic domain
PFam PF00364 Biotin/lipoyl attachment
Superfamily SSF51230 Single hybrid motif
Superfamily SSF52777

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 3 15 11 28
disruptive 7 35 21 62
missense 7 34 21 59

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 5 34 18 27
disruptive 14 72 41 72
missense 12 65 36 61

PlasmoDB Total SNPs: 89

Non-coding: 67 | Synonymous: 11 | Nonsynonymous: 11 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
25116855 Redox-dependent lipoylation of mitochondrial proteins in Plasmodium falciparum. Afanador GA, Matthews KA, ..., Prigge ST 10.1111/mmi.12753
33906926 Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH Sinha A, Baumgarten S, ..., Scherf A 10.1128/mBio.00661-21
34494883 A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. van Esveld SL, Meerstein-Kessel L, ..., Huynen MA 10.1128/mSphere.00614-21