Product Description: dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.1.61 (Dihydrolipoyllysine-residue succinyltransferase)
Curated GO (PlasmoDB):
Type | GO Term | Name |
---|---|---|
Component | GO:0005739 | mitochondrion |
Function | GO:0003723 | RNA binding |
Function | GO:0004149 | dihydrolipoyllysine-residue succinyltransferase activity |
Process | GO:0006103 | 2-oxoglutarate metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
Stage | LR class | MCA mean | MCA prop. zeros |
---|---|---|---|
Sporozoite | expressed | N/A | N/A |
Ring | possibly expressed | 0.05 | 0.97 |
Trophozoite | expressed | 0.41 | 0.75 |
Schizont | expressed | 0.08 | 0.95 |
Gametocyte | expressed | 0.31 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1320800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.398 | #Insertions: 3
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1419100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1419100)
Modification: Disrupted | RMgm-3829
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IEA6
"Best" AlphaFill ligand hit: CAO (oxidized coenzyme a, Local RMSD=0.15) with 1EAD (Global RMSD=1.40)
BRENDA EC Inhibitors:
EC # | Name | EC Inhibitors |
---|---|---|
2.3.1.61 | dihydrolipoyllysine-residue succinyltransferase | coenzyme Q02-(4-fluorophenyl)-5-(methanesulfonyl)-1,3,4-oxadiazole2-(ethanesulfonyl)-5-(4-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-1,3,4-oxadiazole2-(methanesulfonyl)-5-(4-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-1,3,4-oxadiazole2-(methylsulfonyl)-5-(4-fluoro-3-((prop-2-yn-1-yloxy)methyl)-phenyl)-1,3,4-oxadiazole2-(ethanesulfonyl)-5-(4-fluoro-3-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-1,3,4-oxadiazole2-(4-fluoro-3-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-5-(methanesulfonyl)-1,3,4-oxadiazole2-(2,4-dichloro-3-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-5-(ethanesulfonyl)-1,3,4-oxadiazole2-(2,4-dichloro-3-[[(prop-2-yn-1-yl)oxy]methyl]phenyl)-5-(methanesulfonyl)-1,3,4-oxadiazole |
Orthology to BindingDB Entries:
UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
---|---|---|---|---|
P9WIS7 | Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex | OMA, OrthoMCL | US9073941, 101US9073941, 102US9073941, 28US9073941, 28... |
Ortholog Group (OrthoMCL): OG6_101182
Most Similar Human Ortholog: P36957
TM-align score: 0.75 | RMSD: 3.52
Seq Identity: 0.46 | Length: 357 / 421
All Human Orthologs (OrthoMCL):
Gene ID | Description |
---|---|
ENSG00000119689 | dihydrolipoamide S-succinyltransferase |
Protein Length: 421 | Molecular Weight (kDa): 47.631
UniProt ID(s): Q8IEA6
PDB ID(s): None
Isoelectric Point: 6.74
Protein Domain Annotations:
Source | Family ID | Description |
---|---|---|
InterPro | IPR000089 | Biotin/lipoyl attachment |
InterPro | IPR001078 | 2-oxoacid dehydrogenase acyltransferase, catalytic domain |
InterPro | IPR006255 | Dihydrolipoamide succinyltransferase |
InterPro | IPR011053 | Single hybrid motif |
PFam | PF00198 | 2-oxoacid dehydrogenase acyltransferase, catalytic domain |
PFam | PF00364 | Biotin/lipoyl attachment |
Superfamily | SSF51230 | Single hybrid motif |
Superfamily | SSF52777 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 3 | 15 | 11 | 28 |
disruptive | 7 | 35 | 21 | 62 |
missense | 7 | 34 | 21 | 59 |
Any inclusion in genotype call
variant type | common | rare | doubleton | singleton |
---|---|---|---|---|
synonymous | 5 | 34 | 18 | 27 |
disruptive | 14 | 72 | 41 | 72 |
missense | 12 | 65 | 36 | 61 |
PlasmoDB Total SNPs: 89
Non-coding: 67 | Synonymous: 11 | Nonsynonymous: 11 | Stop Codon: 0
PMID | Title | Authors | DOI/Link |
---|---|---|---|
25116855 | Redox-dependent lipoylation of mitochondrial proteins in Plasmodium falciparum. | Afanador GA, Matthews KA, ..., Prigge ST | 10.1111/mmi.12753 |
33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |