PF3D7_1321700 (SF1)

Genome location: Pf3D7_13_v3:901,750..906,652(+)

Genome classification: Core

Function and Localization

Product Description: splicing factor 1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003729	mRNA binding
Process	GO:0008380	RNA splicing
Process	GO:0000375	RNA splicing, via transesterification reactions

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.41	0.42
Trophozoite	expressed	1.80	0.23
Schizont	expressed	0.46	0.74
Gametocyte	expressed	1.40	0.34

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1321700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 1.51 | #Insertions: 3

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1337100)

Relative Growth Rate: 0.13 ± 0.30
Confidence: 3.78

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE99

"Best" AlphaFill ligand hit: CPQ (n,n-bis(3-d-gluconamidopropyl)deoxycholamide, Local RMSD=1.06) with 3VAF (Global RMSD=15.15)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101917

Most Similar Human Ortholog: G3V2B4

TM-align score: 0.84 | RMSD: 1.39

Seq Identity: 0.26 | Length: 50 / 864

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000100461	RNA binding motif protein 23
ENSG00000131051	RNA binding motif protein 39

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	20	41	30	57
disruptive	63	147	76	157
missense	29	99	58	114

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	28	92	42	52
disruptive	101	245	112	232
missense	47	174	85	132

PlasmoDB Total SNPs: 237

Non-coding: 131 | Synonymous: 73 | Nonsynonymous: 33 | Stop Codon: 0

Protein Information

Protein Length: 864 | Molecular Weight (kDa): 100.943

UniProt ID(s): Q8IE99

PDB ID(s): None

Isoelectric Point: 5.34

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000504	RNA recognition motif domain
InterPro	IPR006509	Splicing factor, RBM39-like
InterPro	IPR029123	Splicing factor RBM39, linker
InterPro	IPR035979	RNA-binding domain superfamily
PFam	PF00076	RNA recognition motif domain
PFam	PF15519	Splicing factor RBM39, linker
Superfamily	SSF54928	RNA-binding domain superfamily