PF3D7_1321800

Genome location: Pf3D7_13_v3:907,364..910,306(+)

Genome classification: Core

Function and Localization

Product Description: protein transport protein SFT2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016020	membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.36	0.82
Trophozoite	expressed	0.18	0.89
Schizont	expressed	0.11	0.92
Gametocyte	expressed	0.11	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1321800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.21 | MFS: -2.518 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1337200)

Relative Growth Rate: 0.94 ± 0.13
Confidence: 5.40

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1337200)

Modification: Disrupted | RMgm-3577

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE98

"Best" AlphaFill ligand hit: PX4 (1,2-dimyristoyl-sn-glycero-3-phosphocholine, Local RMSD=0.06) with 8DIZ (Global RMSD=13.35)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103301

Most Similar Human Ortholog: Q587I9

TM-align score: 0.46 | RMSD: 4.41

Seq Identity: 0.19 | Length: 140 / 259

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000173349	SFT2 domain containing 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	11	10	22
disruptive	0	16	12	36
missense	0	15	11	33

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	25	19	18
disruptive	3	41	34	59
missense	2	30	31	43

PlasmoDB Total SNPs: 179

Non-coding: 169 | Synonymous: 8 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 259 | Molecular Weight (kDa): 29.267

UniProt ID(s): Q8IE98

PDB ID(s): None

Isoelectric Point: 8.89

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007305	Vesicle transport protein, Got1/SFT2-like
InterPro	IPR011691	Vesicle transport protein SFT2
InterPro	IPR036259	MFS transporter superfamily
PFam	PF04178	Vesicle transport protein, Got1/SFT2-like
Superfamily	SSF103473	MFS transporter superfamily