About Gene List

PF3D7_1322000

Genome location: Pf3D7_13_v3:914,465..918,899(+)

Genome classification: Core

Function and Localization

Product Description: nucleoside-diphosphatase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 3.6.1.6 (Nucleoside diphosphate phosphatase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020036 Maurer's cleft
Component GO:0005788 endoplasmic reticulum lumen
Component GO:0016020 membrane

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.11 0.94
Trophozoite expressed 0.43 0.75
Schizont expressed 0.25 0.84
Gametocyte expressed 0.24 0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1322000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.127 | MFS: -2.943 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1337400)

  • Relative Growth Rate: 0.10 ± 0.21
  • Confidence: 4.51

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IE96

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.06) with 4BRF (Global RMSD=5.05)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.6.1.6 nucleoside diphosphate phosphatase ATPADPUDPAMPGDPNEMIMPEDTAGDPbetaSmenadioneGTPgammaSsuperoxide...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_154019

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 3 23 18 24
disruptive 39 59 21 63
missense 21 40 17 52

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 6 46 22 37
disruptive 52 103 56 149
missense 30 68 40 94

PlasmoDB Total SNPs: 257

Non-coding: 215 | Synonymous: 16 | Nonsynonymous: 26 | Stop Codon: 0

Protein Information

Protein Length: 565 | Molecular Weight (kDa): 66.2

UniProt ID(s): Q8IE96

PDB ID(s): None

Isoelectric Point: 6.17

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000407 Nucleoside phosphatase GDA1/CD39
PFam PF01150 Nucleoside phosphatase GDA1/CD39
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
11152613 Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. Krogh A, Larsson B, von Heijne G, Sonnhammer EL 10.1006/jmbi.2000.4315
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
19294420 Return to sender: use of Plasmodium ER retrieval sequences to study proteintransport in the infected erythrocyte and predict putative ER protein families. Kulzer S, Gehde N, Przyborski JM 10.1007/s00436-009-1397-x
31631203 A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. Das D, Krishnan SR, Roy A, Bulusu G 10.1039/c9mo00124g